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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-4-23
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pubmed:abstractText |
Several diseases are characterized by chronic inflammation, a condition frequently associated with angiogenesis and fibrogenesis that account for the development of granulation tissue. Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) is a crucial modulator of intracellular prosurvival signaling pathways and is implicated in the pathogenesis of inflammatory process. In this study, we have investigated the role of NF-kappaB in the angiogenic and fibrogenic response induced by lambda-carrageenin in a rat model of chronic inflammation at 1, 3, and 5 days. The subcutaneous implant of lambda-carrageenin-soaked sponges in rat induced a time-related increase of granulation tissue formation accompanied by intense neovascularization. These lambda-carrageenin-induced changes were significantly reduced by coinjection of wild-type oligodeoxynucleotide (WT ODN) decoy to NF-kappaB. Molecular, morphological, and ultrastructural analysis performed on whole granulation tissue demonstrated: (1) inhibition of NF-kappaB/DNA binding activity; (2) downregulation of cyclooxygenase-2, matrix metalloproteinase-9, tumor necrosis factor-alpha, and vascular endothelial growth factor; (3) upregulation of thrombospondin (TSP)-1 at 1 day and TSP-2 at 5 days; and (4) increase in Bax to Bcl-2 ratio. Our findings show that the blockade of NF-kappaB activation by WT ODN decoy prevents the development of granulation tissue induced by lambda-carrageenin-soaked sponge implant upregulating Bax as well as TSP-1 and TSP-2 expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bax protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Carrageenan,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombospondin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombospondins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/thrombospondin 2
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1432-1440
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
481-92
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pubmed:dateRevised |
2011-7-8
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pubmed:meshHeading |
pubmed-meshheading:19189070-Animals,
pubmed-meshheading:19189070-Blotting, Western,
pubmed-meshheading:19189070-Carrageenan,
pubmed-meshheading:19189070-Cyclooxygenase 2,
pubmed-meshheading:19189070-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:19189070-Fibroblasts,
pubmed-meshheading:19189070-Immunohistochemistry,
pubmed-meshheading:19189070-Inflammation,
pubmed-meshheading:19189070-Male,
pubmed-meshheading:19189070-Matrix Metalloproteinase 9,
pubmed-meshheading:19189070-Microscopy, Electron, Scanning,
pubmed-meshheading:19189070-Microscopy, Electron, Transmission,
pubmed-meshheading:19189070-NF-kappa B,
pubmed-meshheading:19189070-Oligonucleotide Probes,
pubmed-meshheading:19189070-Protein Binding,
pubmed-meshheading:19189070-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:19189070-Rats,
pubmed-meshheading:19189070-Rats, Wistar,
pubmed-meshheading:19189070-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19189070-Thrombospondin 1,
pubmed-meshheading:19189070-Thrombospondins,
pubmed-meshheading:19189070-Tumor Necrosis Factor-alpha,
pubmed-meshheading:19189070-Up-Regulation,
pubmed-meshheading:19189070-Vascular Endothelial Growth Factor A,
pubmed-meshheading:19189070-bcl-2-Associated X Protein
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pubmed:year |
2009
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pubmed:articleTitle |
NF-kappaB blockade upregulates Bax, TSP-1, and TSP-2 expression in rat granulation tissue.
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pubmed:affiliation |
Dipartimento di Farmacologia Sperimentale, Facoltà di Scienze Biotecnologiche, Università degli Studi di Napoli Federico II, Via D. Montesano, 49, 80131, Naples, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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