Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-4-1
pubmed:abstractText
The Puu pathway is a putrescine utilization pathway involving gamma-glutamyl intermediates. The genes encoding the enzymes of the Puu pathway form a gene cluster, the puu gene cluster, and puuP is one of the genes in this cluster. In Escherichia coli, three putrescine importers, PotFGHI, PotABCD, and PotE, were discovered in the 1990s and have been studied; however, PuuP had not been discovered previously. This paper shows that PuuP is a novel putrescine importer whose kinetic parameters are equivalent to those of the polyamine importers discovered previously. A puuP(+) strain absorbed up to 5 mM putrescine from the medium, but a DeltapuuP strain did not. E. coli strain MA261 has been used in previous studies of polyamine transporters, but PuuP had not been identified previously. It was revealed that the puuP gene of MA261 was inactivated by a point mutation. When E. coli was grown on minimal medium supplemented with putrescine as the sole carbon or nitrogen source, only PuuP among the polyamine importers was required. puuP was expressed strongly when putrescine was added to the medium or when the puuR gene, which encodes a putative repressor, was deleted. When E. coli was grown in M9-tryptone medium, PuuP was expressed mainly in the exponential growth phase, and PotFGHI was expressed independently of the growth phase.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-10814501, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-10829079, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-11788703, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-12174945, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-12617754, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-14702302, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-14756784, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-15590624, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-1584788, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-16011745, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-16023116, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-16109926, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-16428314, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-16499623, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-18495664, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-1939142, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-2249996, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-2570061, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-2887543, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-3038334, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-3087344, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-3157043, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-371962, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-6206782, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-7754371, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-8416922, http://linkedlifedata.com/resource/pubmed/commentcorrection/19181795-9045651
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1098-5530
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
191
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2776-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
The putrescine Importer PuuP of Escherichia coli K-12.
pubmed:affiliation
Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't