Linked Life Data

19181216

Source:http://linkedlifedata.com/resource/pubmed/id/19181216

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10-11
pubmed:dateCreated
2009-2-2
pubmed:abstractText
Rat small hepatocytes (SHs) are committed progenitor cells that can differentiate into mature hepatocytes and can selectively proliferate in serum-free medium when they are cultured on hyaluronic acid (HA)-coated dishes. In this study we examined the separation of human SHs from adult human livers. We obtained liver tissues from the resected liver of 16 patients who underwent hepatic resections. Extracted liver specimens were clearly separate from the tumor regions with sufficient margins. Hepatic cells were isolated using the modified method of two-step collagenase perfusion. A low-speed centrifugation was performed and cells in the supernatant were finally cultured on HA-coated dishes in serum-free DMEM/F12 medium including nicotinamide, EGF, and HGF. Small-sized hepatocytes selectively proliferated to form colonies and many colonies continued growing for more than 3 weeks. The average number of cells in a colony was 38.6 +/- 18.0, 79.0 +/- 54.0, and 101.5 +/- 115.7 at day 7, 14, and 21, respectively. About 0.04% of plated cells could form an SH colony. Immunocytochemistry showed that the cells forming a colony were positive for albumin, transferrin, keratin 8, and CD44. The results of RT-PCR showed that colony-forming cells expressed albumin, transferrin, alpha1-antitrypsin, fibrinogen, glutamine synthetase, many cytochrome P450s, and liver-enriched transcription factors (HNF3alpha, HNF4alpha, C/EBPalpha, and C/EBPbeta). Furthermore, the cells expressed not only the genes of hepatic differentiated functions but also those of both hepatic stem cell marker (Thy1.1, EpCAM, AFP) and SH marker (CD44, D6.1A, BRI3). Albumin secretion into culture medium was also observed. Our results demonstrate the existence of hepatocyte progenitor cells in human adult livers, and the cells can grow in a serum-free medium on HA-coated dishes. Human SHs may be a useful source for cell transplantation as well as pharmaceutical and toxicological investigations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0963-6897
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1221-30
pubmed:meshHeading
pubmed-meshheading:19181216-Adult, pubmed-meshheading:19181216-Aged, pubmed-meshheading:19181216-Aged, 80 and over, pubmed-meshheading:19181216-Albumins, pubmed-meshheading:19181216-Cell Culture Techniques, pubmed-meshheading:19181216-Cell Proliferation, pubmed-meshheading:19181216-Cells, Cultured, pubmed-meshheading:19181216-Coated Materials, Biocompatible, pubmed-meshheading:19181216-Culture Media, Serum-Free, pubmed-meshheading:19181216-Cytochrome P-450 Enzyme System, pubmed-meshheading:19181216-Female, pubmed-meshheading:19181216-Hepatocytes, pubmed-meshheading:19181216-Humans, pubmed-meshheading:19181216-Hyaluronic Acid, pubmed-meshheading:19181216-Liver, pubmed-meshheading:19181216-Male, pubmed-meshheading:19181216-Middle Aged, pubmed-meshheading:19181216-Specimen Handling, pubmed-meshheading:19181216-Stem Cells
pubmed:year
2008
pubmed:articleTitle
Proliferation of hepatocyte progenitor cells isolated from adult human livers in serum-free medium.
pubmed:affiliation
Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies