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pubmed-article:19180078pubmed:abstractTextThis protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines. Once an existing model is available, reference-based alignment can be used, a procedure that can be iterated. Also described is supervised classification, a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist.lld:pubmed
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pubmed-article:19180078pubmed:articleTitleSPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs.lld:pubmed
pubmed-article:19180078pubmed:affiliationWadsworth Center, Empire State Plaza, Albany, New York 12201-0509, USA.lld:pubmed
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