Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-1-30
pubmed:abstractText
This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines. Once an existing model is available, reference-based alignment can be used, a procedure that can be iterated. Also described is supervised classification, a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-10591646, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-10600563, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-10721991, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-11472089, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-12093756, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-12176389, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-12425301, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-12524193, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-15065676, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-15450297, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-1580010, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-16621601, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-16973604, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-17055743, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-17086193, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-17179934, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-17360628, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-17446867, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-3058896, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-32653, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-3302267, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-7268930, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-7688427, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-8742718, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-8742743, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-8921628, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-9169230, http://linkedlifedata.com/resource/pubmed/commentcorrection/19180078-9535885
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1750-2799
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1941-74
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs.
pubmed:affiliation
Wadsworth Center, Empire State Plaza, Albany, New York 12201-0509, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural