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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-1-30
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pubmed:abstractText |
The limbus is enriched with the stem cells of corneal epithelium. Auto- and allograft limbal transplantations are effective in restoring the corneal epithelium and inhibiting inflammation and neovascularization. Preserved human amniotic membrane (AM) is now widely used as a substrate for ocular surface reconstruction. The combination of limbal and AM transplantation has been shown to improve the surgical outcome in patients with total limbal stem cell deficiency (LSCD). The purpose of this study was to compare the expression of putative stem cell markers ATP binding cassette protein (ABCG2) and keratinocyte stem cell marker: p63 and differentiation markers. (connexin 43 and keratin 3 / keratin 12) on the limbal epithelial cells cultured over the denuded AM with and without the 3T3 murine fibroblast cells as feeder layer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0971-5916
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
616-22
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pubmed:meshHeading |
pubmed-meshheading:19179682-3T3 Cells,
pubmed-meshheading:19179682-ATP-Binding Cassette Transporters,
pubmed-meshheading:19179682-Animals,
pubmed-meshheading:19179682-Blotting, Western,
pubmed-meshheading:19179682-Cells, Cultured,
pubmed-meshheading:19179682-Connexin 43,
pubmed-meshheading:19179682-Epithelium, Corneal,
pubmed-meshheading:19179682-Humans,
pubmed-meshheading:19179682-Immunohistochemistry,
pubmed-meshheading:19179682-Membrane Proteins,
pubmed-meshheading:19179682-Mice,
pubmed-meshheading:19179682-Neoplasm Proteins,
pubmed-meshheading:19179682-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19179682-Stem Cells
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pubmed:year |
2008
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pubmed:articleTitle |
Influence of feeder layer on the expression of stem cell markers in cultured limbal corneal epithelial cells.
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pubmed:affiliation |
L & T Department of Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, Chennai, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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