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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
27
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pubmed:dateCreated |
1991-10-29
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72399,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72400,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72401,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72402,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72403,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72404,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72405,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72406,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72407,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72408,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72409,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M72410
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pubmed:abstractText |
We have constructed a plasmid, pLTR100, which contains human interleukin 2 receptor light (IL-2R L) chain cDNA in the inverted orientation relative to the upstream SV40 promoter. The cDNA segment is flanked by the immunoglobulin gene recombination signal sequences so that the cDNA segment can invert and the human IL-2R L chain is subsequently expressed under the control of the SV40 promoter. A murine pre-B cell line, 38B9, transfected with pLTR100 began to express the human IL-2R L chain on the cell surface. The frequency of human IL-2R L chain positive cells increased almost linearly up to 50% for 60 days of culture after transfection. Southern blot analysis and sequencing of the DNA fragments at the recombination junction confirmed that the cDNA segment was inverted in a signal sequence-dependent manner by the variable-diversity-joining recombination process. Transgenic mice bearing the recombination substrate DNA similar to pLTR100 expressed the human IL-2 L chain in the spleen, thymus, and bone marrow, but not in the other tissues examined at the detectable level. Both IgM- and CD3-positive cells expressed the human IL-2R L chain, indicating that this artificial DNA can serve as a substrate for recombination both in B- and T-cells and that another DNA segment may be necessary to confer the cell-type specificity on the substrate DNA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
266
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
18387-94
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1917962-Animals,
pubmed-meshheading:1917962-B-Lymphocytes,
pubmed-meshheading:1917962-Base Sequence,
pubmed-meshheading:1917962-Blotting, Southern,
pubmed-meshheading:1917962-Cell Line,
pubmed-meshheading:1917962-DNA,
pubmed-meshheading:1917962-Humans,
pubmed-meshheading:1917962-Immunoglobulins,
pubmed-meshheading:1917962-Mice,
pubmed-meshheading:1917962-Mice, Transgenic,
pubmed-meshheading:1917962-Molecular Sequence Data,
pubmed-meshheading:1917962-Plasmids,
pubmed-meshheading:1917962-Polymerase Chain Reaction,
pubmed-meshheading:1917962-Promoter Regions, Genetic,
pubmed-meshheading:1917962-Receptors, Interleukin-2,
pubmed-meshheading:1917962-Recombination, Genetic,
pubmed-meshheading:1917962-Spleen,
pubmed-meshheading:1917962-Substrate Specificity,
pubmed-meshheading:1917962-Thymus Gland,
pubmed-meshheading:1917962-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
Recombination of exogenous interleukin 2 receptor gene flanked by immunoglobulin recombination signal sequences in a pre-B cell line and transgenic mice.
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pubmed:affiliation |
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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