Linked Life Data

19177654

Source:http://linkedlifedata.com/resource/pubmed/id/19177654

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-1-28
pubmed:abstractText
Here we describe the design, synthesis, and characterization of new, metal-functionalized, amphiphilic diblock copolymers for molecular recognition. Polybutadiene-block-polyethylenoxide copolymers were synthesized by living anionic polymerization and end group functionalized with nitrilotriacetic acid and tris(nitrilotriacetic acid). After complexation with nickel and copper, these groups are known to selectively bind to oligohistidine residues of proteins. The polymers were characterized by 1H NMR spectroscopy, size exclusion chromatography, electron paramagnetic resonance, and UV-vis spectroscopy. Mixtures of these polymers with the respective nonfunctionalized block copolymers self-assemble in aqueous solution into vesicular structures with a controlled density of the metal complex end-groups on their surface. The accessibility of these binding sites was tested using maltose binding protein carrying a terminal decahistidine moiety and His-tagged enhanced green fluorescent protein as model systems. Fluorescence correlation spectroscopy clearly showed a significant and selective binding of these proteins to the vesicle surface.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0743-7463
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1122-30
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Amphiphilic diblock copolymers for molecular recognition: metal-nitrilotriacetic acid functionalized vesicles.
pubmed:affiliation
Department of Chemistry, University of Basel, Klingelbergstrasse 80, CH-4056 Basel, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't