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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-5-1
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pubmed:abstractText |
Cathepsin E is an endo-lysosomal aspartic proteinase exclusively present in immune system cells. Previous studies have shown that cathepsin E-deficient (CatE(-/-)) mice display aberrant immune responses such as atopic dermatitis and higher susceptibility to bacterial infection. However, the mechanisms underlying abnormal immune responses induced by cathepsin E deficiency are still unclear. In this study, we found that the cell-surface levels of chemotactic receptors, including chemokine receptor (CCR)-2 and N-formyl peptide receptors (FPRs), were clearly diminished in CatE(-/-)macrophages compared with those in wild-type cells. Consistently, chemotaxis of CatE(-/-)macrophages to MCP-1 and N-formyl-methionyl-leucyl-phenylalanine was also decreased. Similar to the chemotactic receptors, the surface expressions of the adhesion receptors CD18 (integrin beta(2)) and CD 29 (integrin beta(1)) in CatE(-/-) macrophages were significantly decreased, thereby reducing cell attachment of CatE(-/-) macrophages. These results indicate that the defects in chemotaxis and cell adhesion are likely to be involved in the imperfect function of CatE(-/-)macrophages.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin E,
http://linkedlifedata.com/resource/pubmed/chemical/Dextrans,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Lamp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lysosomal-Associated Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Lysosome-Associated Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Thioglycolates,
http://linkedlifedata.com/resource/pubmed/chemical/fluorescein isothiocyanate dextran,
http://linkedlifedata.com/resource/pubmed/chemical/methionyl-leucyl-phenylalanine
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1756-2651
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
145
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
565-73
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pubmed:meshHeading |
pubmed-meshheading:19174547-Animals,
pubmed-meshheading:19174547-Cathepsin E,
pubmed-meshheading:19174547-Cell Adhesion,
pubmed-meshheading:19174547-Cell Count,
pubmed-meshheading:19174547-Chemotaxis,
pubmed-meshheading:19174547-Dextrans,
pubmed-meshheading:19174547-Endocytosis,
pubmed-meshheading:19174547-Fibronectins,
pubmed-meshheading:19174547-Flow Cytometry,
pubmed-meshheading:19174547-Fluorescein-5-isothiocyanate,
pubmed-meshheading:19174547-Lysosomal-Associated Membrane Protein 2,
pubmed-meshheading:19174547-Lysosome-Associated Membrane Glycoproteins,
pubmed-meshheading:19174547-Macrophages, Peritoneal,
pubmed-meshheading:19174547-Mice,
pubmed-meshheading:19174547-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:19174547-Receptors, Cell Surface,
pubmed-meshheading:19174547-Thioglycolates
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pubmed:year |
2009
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pubmed:articleTitle |
Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages.
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pubmed:affiliation |
Department of Dental Pharmacology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. tsuta@nagasaki-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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