Linked Life Data

19167844

Source:http://linkedlifedata.com/resource/pubmed/id/19167844

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2009-4-24
pubmed:abstractText
Human psoriasin (S100A7) has originally been described as a member of the family of S100 calcium-binding proteins which is overexpressed in patients suffering from psoriasis. The bovine homolog was first identified as a cow-derived respiratory allergen. As Escherichia coli mastitis is a common problem in dairy cattle, and human psoriasin was found to exhibit antimicrobial activity preferentially against E. coli, we examined whether the bovine mRNA is expressed in the mammary gland. To demonstrate the antimicrobial activity of bovine psoriasin, we isolated cDNA from the udder, cloned the bovine psoriasin gene in a bacterial expression vector, and the recombinant protein was expressed in BL21 cells. The in vitro antibacterial activity was tested by performing microdilution susceptibility tests and radial diffusion assays with eight different bacterial strains, thereof three different E. coli strains, and one yeast. The antimicrobial activity of the recombinant bovine psoriasin is comparable with human psoriasin and also limited to E. coli. Psoriasin appears to be a part of the local host defense mechanism in the udder, is a putative candidate for a cow-specific factor influencing mastitis susceptibility, and a possible alternative to conventional antibiotics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0378-1135
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
335-40
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:19167844-Amino Acid Sequence, pubmed-meshheading:19167844-Animals, pubmed-meshheading:19167844-Calcium-Binding Proteins, pubmed-meshheading:19167844-Cattle, pubmed-meshheading:19167844-Chromatography, Gel, pubmed-meshheading:19167844-Circular Dichroism, pubmed-meshheading:19167844-Cloning, Molecular, pubmed-meshheading:19167844-DNA, Complementary, pubmed-meshheading:19167844-Female, pubmed-meshheading:19167844-Humans, pubmed-meshheading:19167844-Microbial Sensitivity Tests, pubmed-meshheading:19167844-Molecular Sequence Data, pubmed-meshheading:19167844-RNA, pubmed-meshheading:19167844-Recombinant Proteins, pubmed-meshheading:19167844-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19167844-S100 Proteins, pubmed-meshheading:19167844-Sequence Alignment
pubmed:year
2009
pubmed:articleTitle
Antimicrobial activity of bovine psoriasin.
pubmed:affiliation
Institut für Tierwissenschaften, Rheinische Friedrich-Wilhelms-Universität Bonn, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't