Linked Life Data

19167430

Source:http://linkedlifedata.com/resource/pubmed/id/19167430

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-3-16
pubmed:abstractText
This study examined the effect of two dissection techniques on the quality of human brain specimens. Frozen cerebellar samples were obtained from postmortem brains of 10 subjects free from neurological and psychiatric disease. These tissues were tested for RNA and DNA concentration and quality after being dissected with either an electric dental drill or a small handsaw. RNA and DNA were extracted separately from each sample, and the concentrations and quality of each were measured. We found that dissection technique does not significantly affect RNA or DNA quality/yield. RNA and DNA yields, as well as RNA integrity showed no significant differences between the two dissection techniques. Therefore, these results support the use of a high-speed hand-held electric dental drill as an efficient and anatomically precise means of human brain dissection without compromising tissue quality.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1872-678X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
30
pubmed:volume
179
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
68-70
pubmed:dateRevised
2011-9-28
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
A comparison of human brain dissection by drill versus saw on nucleic acid quality.
pubmed:affiliation
National Institutes of Mental Health, Clinical Brain Disorders Branch, Bethesda, MD 20892, USA. buerleinr@mail.nih.gov
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural