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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2009-2-16
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pubmed:abstractText |
An efficient synthesis of a 5-fluorouracil-cephalosporin prodrug is described for use against colorectal and other cancers in antibody and gene-directed therapies. The compound shows stability in aqueous media until specifically activated by beta-lactamase (betaL). The kinetic parameters of the 5-fluorouracil-cephalosporin conjugate were determined in the presence of Enterobacter cloacae P99 betaL (ECl betaL) revealing a K(m)=95.4 microM and V(max)=3.21 microMol min(-1) mg(-1). The data compare favorably to related systems that have been reported and enable testing of this prodrug against cancer cell lines in vitro and in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-11241346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-11959554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-12369965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-12729642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-15624952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-16220906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-17109545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-5432862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-7731023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-9276025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-9383424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-9417030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19167216-9554883
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1464-3405
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1261-3
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pubmed:dateRevised |
2011-2-2
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pubmed:meshHeading |
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pubmed:year |
2009
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pubmed:articleTitle |
Design and synthesis of a beta-lactamase activated 5-fluorouracil prodrug.
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pubmed:affiliation |
Department of Chemistry, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21212, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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