Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-2-23
pubmed:abstractText
The chicken DT40 cell line is widely used for gene knock-outs. We attempted to introduce a polymerase-dead point mutation into Polkappa, a polymerase for translesion DNA synthesis, taking advantage of the highly efficient targeted integration in DT40 cells. The resulting cells (REV3(-/-)POLK(/)(pol-dead)) proliferated with the same kinetics as the parental REV3(-/-) cells. Though the mock-treated REV3(-/-)POLK(/)(mock) cells showed the same sensitivity as the parental REV3(-/-) cells to methyl methanesulfonate, the REV3(-/-)POLK(/)(pol-dead) cells demonstrated the same sensitivity as the REV3(-/-)POLK(/-) double knock-out cells. This implies that the presence of the polymerase-dead Polkappa does not interfere with other polymerases repairing monoalkylation damage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-3468
pubmed:author
pubmed:issnType
Electronic
pubmed:day
18
pubmed:volume
583
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
661-4
pubmed:meshHeading
pubmed-meshheading:19166845-Animals, pubmed-meshheading:19166845-Asparagine, pubmed-meshheading:19166845-Binding Sites, pubmed-meshheading:19166845-Cell Line, pubmed-meshheading:19166845-Cell Proliferation, pubmed-meshheading:19166845-Chickens, pubmed-meshheading:19166845-Clone Cells, pubmed-meshheading:19166845-DNA Damage, pubmed-meshheading:19166845-DNA-Directed DNA Polymerase, pubmed-meshheading:19166845-Exons, pubmed-meshheading:19166845-Gene Knockout Techniques, pubmed-meshheading:19166845-Kinetics, pubmed-meshheading:19166845-Methyl Methanesulfonate, pubmed-meshheading:19166845-Mutagens, pubmed-meshheading:19166845-Point Mutation, pubmed-meshheading:19166845-Recombination, Genetic, pubmed-meshheading:19166845-Sensitivity and Specificity, pubmed-meshheading:19166845-Transfection, pubmed-meshheading:19166845-Vertebrates
pubmed:year
2009
pubmed:articleTitle
Introduction and characterization of a polymerase-dead point mutation into the POLK gene in vertebrates.
pubmed:affiliation
Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo, 113-8510 Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't