Source:http://linkedlifedata.com/resource/pubmed/id/19166845
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2009-2-23
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pubmed:abstractText |
The chicken DT40 cell line is widely used for gene knock-outs. We attempted to introduce a polymerase-dead point mutation into Polkappa, a polymerase for translesion DNA synthesis, taking advantage of the highly efficient targeted integration in DT40 cells. The resulting cells (REV3(-/-)POLK(/)(pol-dead)) proliferated with the same kinetics as the parental REV3(-/-) cells. Though the mock-treated REV3(-/-)POLK(/)(mock) cells showed the same sensitivity as the parental REV3(-/-) cells to methyl methanesulfonate, the REV3(-/-)POLK(/)(pol-dead) cells demonstrated the same sensitivity as the REV3(-/-)POLK(/-) double knock-out cells. This implies that the presence of the polymerase-dead Polkappa does not interfere with other polymerases repairing monoalkylation damage.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1873-3468
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
18
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pubmed:volume |
583
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
661-4
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pubmed:meshHeading |
pubmed-meshheading:19166845-Animals,
pubmed-meshheading:19166845-Asparagine,
pubmed-meshheading:19166845-Binding Sites,
pubmed-meshheading:19166845-Cell Line,
pubmed-meshheading:19166845-Cell Proliferation,
pubmed-meshheading:19166845-Chickens,
pubmed-meshheading:19166845-Clone Cells,
pubmed-meshheading:19166845-DNA Damage,
pubmed-meshheading:19166845-DNA-Directed DNA Polymerase,
pubmed-meshheading:19166845-Exons,
pubmed-meshheading:19166845-Gene Knockout Techniques,
pubmed-meshheading:19166845-Kinetics,
pubmed-meshheading:19166845-Methyl Methanesulfonate,
pubmed-meshheading:19166845-Mutagens,
pubmed-meshheading:19166845-Point Mutation,
pubmed-meshheading:19166845-Recombination, Genetic,
pubmed-meshheading:19166845-Sensitivity and Specificity,
pubmed-meshheading:19166845-Transfection,
pubmed-meshheading:19166845-Vertebrates
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pubmed:year |
2009
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pubmed:articleTitle |
Introduction and characterization of a polymerase-dead point mutation into the POLK gene in vertebrates.
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pubmed:affiliation |
Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo, 113-8510 Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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