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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-3-30
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pubmed:abstractText |
The extracellular isoform of superoxide dismutase (EC-SOD, Sod3) plays a protective role against various diseases and injuries mediated by oxidative stress. To investigate the pathophysiological roles of EC-SOD, we generated tetracycline-inducible Sod3 transgenic mice and directed the tissue-specific expression of transgenes by crossing Sod3 transgenic mice with tissue-specific transactivator transgenics. Double transgenic mice with liver-specific expression of Sod3 showed increased EC-SOD levels predominantly in the plasma as the circulating form, whereas double transgenic mice with neuronal-specific expression expressed higher levels of EC-SOD in hippocampus and cortex with intact EC-SOD as the dominant form. EC-SOD protein levels also correlated well with increased SOD activities in double transgenic mice. In addition to enabling tissue-specific expression, the transgene expression can be quickly turned on and off by doxycycline supplementation in the mouse chow. This mouse model, thus, provides the flexibility for on-off control of transgene expression in multiple target tissues.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-10051199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-10194479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-10341450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-10766812,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-11133496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-11457885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-11861638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-12060579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-12123763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-12471087,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-6487268,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-8572553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-8603917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-8743981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-8855286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-8939850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165829-9728905
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1526-968X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
142-54
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pubmed:dateRevised |
2011-7-20
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pubmed:meshHeading |
pubmed-meshheading:19165829-Animals,
pubmed-meshheading:19165829-Anti-Bacterial Agents,
pubmed-meshheading:19165829-Crosses, Genetic,
pubmed-meshheading:19165829-Doxycycline,
pubmed-meshheading:19165829-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:19165829-Hippocampus,
pubmed-meshheading:19165829-Liver,
pubmed-meshheading:19165829-Mice,
pubmed-meshheading:19165829-Mice, Transgenic,
pubmed-meshheading:19165829-Models, Biological,
pubmed-meshheading:19165829-Organ Specificity,
pubmed-meshheading:19165829-Oxidative Stress,
pubmed-meshheading:19165829-Superoxide Dismutase,
pubmed-meshheading:19165829-Transgenes
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pubmed:year |
2009
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pubmed:articleTitle |
A new mouse model for temporal- and tissue-specific control of extracellular superoxide dismutase.
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pubmed:affiliation |
Department of Neurology and Neurological Sciences, Stanford University, Stanford, California, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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