Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-1-23
pubmed:abstractText
The nuclear lamina is a major obstacle encountered by herpesvirus nucleocapsids in their passage from the nucleus to the cytoplasm (nuclear egress). We found that the human cytomegalovirus (HCMV)-encoded protein kinase UL97, which is required for efficient nuclear egress, phosphorylates the nuclear lamina component lamin A/C in vitro on sites targeted by Cdc2/cyclin-dependent kinase 1, the enzyme that is responsible for breaking down the nuclear lamina during mitosis. Quantitative mass spectrometry analyses, comparing lamin A/C isolated from cells infected with viruses either expressing or lacking UL97 activity, revealed UL97-dependent phosphorylation of lamin A/C on the serine at residue 22 (Ser(22)). Transient treatment of HCMV-infected cells with maribavir, an inhibitor of UL97 kinase activity, reduced lamin A/C phosphorylation by approximately 50%, consistent with UL97 directly phosphorylating lamin A/C during HCMV replication. Phosphorylation of lamin A/C during viral replication was accompanied by changes in the shape of the nucleus, as well as thinning, invaginations, and discrete breaks in the nuclear lamina, all of which required UL97 activity. As Ser(22) is a phosphorylation site of particularly strong relevance for lamin A/C disassembly, our data support a model wherein viral mimicry of a mitotic host cell kinase activity promotes nuclear egress while accommodating viral arrest of the cell cycle.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-10364316, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-10623760, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-10933677, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-11507226, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-11799148, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-11877373, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-12048183, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-12121906, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-12161659, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-12502806, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-12755618, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-12809528, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-12931342, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-14504651, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-14645578, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-15140953, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-15140956, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-15254174, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-15302935, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-15517000, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-15890938, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-15975922, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-16083285, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-16306620, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-16352573, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-16452087, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-16674113, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-16731927, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-16872656, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-16964243, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-17022650, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-17206862, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-17301149, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-17634236, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-17652388, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-17872514, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-18272765, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-18321963, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-18467589, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-18497856, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-1851086, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-18815303, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-2188730, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-2188731, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-2344612, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-2541673, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-6243379, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-7474079, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-8034666, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-8477740, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-8663071, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-8862409, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-8971013, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-8985364, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-8995690, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-9030781, http://linkedlifedata.com/resource/pubmed/commentcorrection/19165338-9557655
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1553-7374
pubmed:author
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