Linked Life Data

19161387

Source:http://linkedlifedata.com/resource/pubmed/id/19161387

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-1-23
pubmed:abstractText
Phosphatidylinositol-4, 5-bisphosphate [PI(4,5)P(2)] has been implicated in the priming of large dense-core vesicles in many secretory cells; however, its role in the Ca(2+)-dependent secretory activity in pituitary cells remains elusive. We assessed the effect of elevated intracellular PI(4,5)P(2) on the kinetics of Ca(2+)-dependent exocytosis, using a whole-cell patch-clamp technique in wild-type mouse melanotrophs from fresh pituitary tissue slices. We found that 1 micromol/L PI(4,5)P(2) significantly increased Ca(2+)-dependent exocytosis of vesicles that need to go through ATP-dependent reactions; however, the exocytosis of release-ready vesicles (ATP-independent release) and voltage-activated Ca(2+) currents remained unaffected. We suggest that PI(4,5)P(2) increases the size of the readily releasable vesicle pool by regulating the effectiveness of vesicular mobilization and fusion in an ATP-dependent manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1749-6632
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
1152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-73
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Phosphatidylinositol-4,5-bisphosphate-dependent facilitation of the ATP-dependent secretory activity in mouse pituitary cells.
pubmed:affiliation
European Neuroscience Institute-Göttingen, Göttingen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't