19160513

Source:http://linkedlifedata.com/resource/pubmed/id/19160513

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0014834, umls-concept:C0314603, umls-concept:C0392762, umls-concept:C0936012, umls-concept:C1704675
pubmed:issue	9
pubmed:dateCreated	2009-1-22
pubmed:abstractText	Large-scale genetic interaction studies provide the basis for defining gene function and pathway architecture. Recent advances in the ability to generate double mutants en masse in Saccharomyces cerevisiae have dramatically accelerated the acquisition of genetic interaction information and the biological inferences that follow. Here we describe a method based on F factor-driven conjugation, which allows for high-throughput generation of double mutants in Escherichia coli. This method, termed genetic interaction analysis technology for E. coli (GIANT-coli), permits us to systematically generate and array double-mutant cells on solid media in high-density arrays. We show that colony size provides a robust and quantitative output of cellular fitness and that GIANT-coli can recapitulate known synthetic interactions and identify previously unidentified negative (synthetic sickness or lethality) and positive (suppressive or epistatic) relationships. Finally, we describe a complementary strategy for genome-wide suppressor-mutant identification. Together, these methods permit rapid, large-scale genetic interaction studies in E. coli.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/GM036278, http://linkedlifedata.com/resource/pubmed/grant/GM62662, http://linkedlifedata.com/resource/pubmed/grant/R01 AI059027-03, http://linkedlifedata.com/resource/pubmed/grant/R01 GM036278-25, http://linkedlifedata.com/resource/pubmed/grant/R01 GM062662-04, http://linkedlifedata.com/resource/pubmed/grant/T32 DE007306-13
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pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/101215604
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Sep
pubmed:issn	1548-7091
pubmed:author	pubmed-author:BrabergHannesH, pubmed-author:CollinsSean RSR, pubmed-author:GrossCarol ACA, pubmed-author:KroganNevan JNJ, pubmed-author:LimBentleyB, pubmed-author:MoriHirotadaH, pubmed-author:NicholsRobert JRJ, pubmed-author:ShalesMichaelM, pubmed-author:SiegeleDeborah ADA, pubmed-author:TakeuchiRikiyaR, pubmed-author:TypasAthanasiosA, pubmed-author:WannerBarry LBL, pubmed-author:WeissmanJonathan SJS, pubmed-author:YamamotoNatsukoN
pubmed:issnType	Print
pubmed:volume	5
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	781-7
pubmed:dateRevised	2011-9-26
pubmed:meshHeading	pubmed-meshheading:19160513-Conjugation, Genetic, pubmed-meshheading:19160513-Escherichia coli, pubmed-meshheading:19160513-Genome, Bacterial, pubmed-meshheading:19160513-Mutation
pubmed:year	2008
pubmed:articleTitle	High-throughput, quantitative analyses of genetic interactions in E. coli.
pubmed:affiliation	Department of Microbiology and Immunology, University of California at San Francisco, 600 16th Street, San Francisco, California 94158, USA.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural