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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-2-23
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pubmed:abstractText |
The bone marrow (BM) microenvironment plays a critical role in malignant cell growth, patient survival and response to chemotherapy in hematologic malignancies. However, the molecular mechanisms of BM stromal cells (BMSCs)-mediated survival of tumor cell remain unclear. In this study, to further evaluate the role of Notch-1 in vivo microenvironment, we investigated the influence of inhibiting Notch-1 pathway by Notch-1 siRNA on Jurkat cells in the co-culture system. We found that Notch-1 signaling in Jurkat cells was further activated by interaction with BMSCs, which inhibited drug-induced apoptosis in Jurkat cells. Notch-1 siRNA down-regulated Notch-1 and restored drug-induced apoptosis in co-cultured Jurkat cells. The possible mechanism of restoration of sensitivity to chemotherapy could be associated with repressed Akt signaling. The results indicated that Notch-1 may be a potential mechanism of BMSCs involvement in the protection of hematopoietic malignant cells from drug-induced apoptosis.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1029-2403
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
270-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:19160126-Antineoplastic Agents,
pubmed-meshheading:19160126-Apoptosis,
pubmed-meshheading:19160126-Bone Marrow Cells,
pubmed-meshheading:19160126-Cell Communication,
pubmed-meshheading:19160126-Coculture Techniques,
pubmed-meshheading:19160126-Down-Regulation,
pubmed-meshheading:19160126-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19160126-Humans,
pubmed-meshheading:19160126-Jurkat Cells,
pubmed-meshheading:19160126-Leukemia, T-Cell,
pubmed-meshheading:19160126-Phosphorylation,
pubmed-meshheading:19160126-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:19160126-RNA, Small Interfering,
pubmed-meshheading:19160126-Receptor, Notch1,
pubmed-meshheading:19160126-Signal Transduction,
pubmed-meshheading:19160126-Stromal Cells,
pubmed-meshheading:19160126-Substrate Specificity,
pubmed-meshheading:19160126-Up-Regulation
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pubmed:year |
2009
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pubmed:articleTitle |
Down-regulation of Notch-1 increases co-cultured Jurkat cell sensitivity to chemotherapy.
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pubmed:affiliation |
Department of Hematology, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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