|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1991-10-28
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Full-length cDNAs coding for the human GM2-activator protein has been isolated and characterized, and its genomic structure studied in two overlapping clones in lambda-EMBL-4 isolated from a human brain genomic library. Two different cDNAs were found that were identical to the 5'-terminus to nt 1311 (counted from the A of the initiation codon, ATG) including the entire protein coding sequence. However, they were entirely dissimilar in the 3'-non-coding sequences. The genomic clones covered 94% of the full-length cDNA sequence. Three introns were found. The last exon spans contiguously the carboxyl terminus of the protein and the entire 3'-untranslated region of one of the two cDNAs with different 3'-ends. The origin of the 3'-portion of the other cDNA clone is not clear at this time.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
9
|
|
pubmed:volume |
289
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
260-4
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:1915857-Base Sequence,
pubmed-meshheading:1915857-Cloning, Molecular,
pubmed-meshheading:1915857-DNA,
pubmed-meshheading:1915857-Escherichia coli,
pubmed-meshheading:1915857-Exons,
pubmed-meshheading:1915857-G(M2) Activator Protein,
pubmed-meshheading:1915857-G(M2) Ganglioside,
pubmed-meshheading:1915857-Genes,
pubmed-meshheading:1915857-Humans,
pubmed-meshheading:1915857-Introns,
pubmed-meshheading:1915857-Molecular Sequence Data,
pubmed-meshheading:1915857-Polymerase Chain Reaction,
pubmed-meshheading:1915857-Proteins,
pubmed-meshheading:1915857-Restriction Mapping
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Characterization of full-length cDNAs and the gene coding for the human GM2 activator protein.
|
|
pubmed:affiliation |
Institut für Organische Chemie und Biochemie, Bonn, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
