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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-2-9
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pubmed:abstractText |
Mammalian forms of the transcription repressor, Kaiso, can reportedly bind methylated DNA and non-methylated CTGCNA motifs. Here we compare the DNA-binding properties of Kaiso from frog, fish and chicken and demonstrate that only the methyl-CpG-binding function of Kaiso is evolutionarily conserved. We present several independent experimental lines of evidence that the phenotypic abnormalities associated with xKaiso-depleted Xenopus laevis embryos are independent of the putative CTGCNA-dependent DNA-binding function of xKaiso. Our analysis suggests that xKaiso does not play a role in the regulation of either xWnt11 or Siamois, key signalling molecules in the Wnt pathway during X. laevis gastrulation. The major phenotypic defects associated with xKaiso depletion are premature transcription activation before the mid-blastula transition and concomitant activation of a p53-dependent cell-death pathway.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-10207085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-10673503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-10769246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-11017081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-11445535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-12087177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-12163405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-12732139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-14651922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-15543138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-15548582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-15797385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-15878828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-15935774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-16354688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-16354691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-16980612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-16990798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-18305009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-18794111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-19158184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-7940754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-8287796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-9032060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-9308964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19158185-9891351
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/XKaiso protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/siamois protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/wnt-11 protein, Xenopus
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0950-1991
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pubmed:author |
pubmed-author:ChekanovNikolaiN,
pubmed-author:DunicanDonncha SDS,
pubmed-author:HackettJamie AJA,
pubmed-author:LiMinghuiM,
pubmed-author:MadejMonika JMJ,
pubmed-author:MeehanRichard RRR,
pubmed-author:PenningsSariS,
pubmed-author:ProkhortchoukAnnaA,
pubmed-author:ProkhortchoukEgorE,
pubmed-author:ReddingtonJames PJP,
pubmed-author:RuzovAlexeyA,
pubmed-author:SavitskayaEkaterinaE
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pubmed:issnType |
Print
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pubmed:volume |
136
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
729-38
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
pubmed-meshheading:19158185-Animals,
pubmed-meshheading:19158185-Animals, Genetically Modified,
pubmed-meshheading:19158185-Apoptosis,
pubmed-meshheading:19158185-Base Sequence,
pubmed-meshheading:19158185-Binding Sites,
pubmed-meshheading:19158185-Chickens,
pubmed-meshheading:19158185-Conserved Sequence,
pubmed-meshheading:19158185-CpG Islands,
pubmed-meshheading:19158185-DNA,
pubmed-meshheading:19158185-DNA Methylation,
pubmed-meshheading:19158185-Gastrulation,
pubmed-meshheading:19158185-Homeodomain Proteins,
pubmed-meshheading:19158185-Humans,
pubmed-meshheading:19158185-Phenotype,
pubmed-meshheading:19158185-Recombinant Fusion Proteins,
pubmed-meshheading:19158185-Repressor Proteins,
pubmed-meshheading:19158185-Signal Transduction,
pubmed-meshheading:19158185-Species Specificity,
pubmed-meshheading:19158185-Takifugu,
pubmed-meshheading:19158185-Wnt Proteins,
pubmed-meshheading:19158185-Xenopus Proteins,
pubmed-meshheading:19158185-Xenopus laevis,
pubmed-meshheading:19158185-Zebrafish,
pubmed-meshheading:19158185-Zebrafish Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
The non-methylated DNA-binding function of Kaiso is not required in early Xenopus laevis development.
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pubmed:affiliation |
Human Genetics Unit, MRC, Western General Hospital, Edinburgh, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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