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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-1-21
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pubmed:abstractText |
The need for reporter lines able to faithfully track Th17 cells in vivo has become an issue of exceptional importance. To address this, we generated a mouse strain in which Cre recombinase is expressed from the IL-17F promoter. Crossing the IL-17F-Cre allele to a conditional enhanced yellow fluorescent protein (EYFP) reporter mouse yielded the IL-17F-Cre(EYFP) strain, in which IL-17F expression is twinned with EYFP in live IL-17F-expressing cells. Although we demonstrate that IL-17F expression is restricted to CD4(+) T cells during experimental autoimmune encephalomyelitis, IL-17F-Cre(EYFP) CD8 T cells robustly expressed IL-17F in response to TGF-beta, IL-6, and IL-23. Fate mapping of IL-17F-expressing reporter T cells revealed a significant down-regulation of Th17 cytokines after homeostatic expansion in RAG1-deficient animals. Despite this loss of effector phenotype, committed Th17 cells were resistant to Foxp3 expression in vitro or in vivo. Thus, the IL-17F-Cre strain furthers our understanding of Th17 biology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1550-6606
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
182
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1237-41
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pubmed:dateRevised |
2010-9-29
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pubmed:meshHeading |
pubmed-meshheading:19155467-Adoptive Transfer,
pubmed-meshheading:19155467-Animals,
pubmed-meshheading:19155467-Bacterial Proteins,
pubmed-meshheading:19155467-CD8-Positive T-Lymphocytes,
pubmed-meshheading:19155467-Cell Differentiation,
pubmed-meshheading:19155467-Cells, Cultured,
pubmed-meshheading:19155467-Encephalomyelitis, Autoimmune, Experimental,
pubmed-meshheading:19155467-Gene Expression Regulation,
pubmed-meshheading:19155467-Genes, Reporter,
pubmed-meshheading:19155467-Humans,
pubmed-meshheading:19155467-Immunophenotyping,
pubmed-meshheading:19155467-Integrases,
pubmed-meshheading:19155467-Interleukin-17,
pubmed-meshheading:19155467-Luminescent Proteins,
pubmed-meshheading:19155467-Mice,
pubmed-meshheading:19155467-Mice, Inbred C57BL,
pubmed-meshheading:19155467-Mice, Inbred DBA,
pubmed-meshheading:19155467-Mice, Transgenic,
pubmed-meshheading:19155467-Proteins,
pubmed-meshheading:19155467-T-Lymphocytes, Regulatory
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pubmed:year |
2009
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pubmed:articleTitle |
Cutting edge: an IL-17F-CreEYFP reporter mouse allows fate mapping of Th17 cells.
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pubmed:affiliation |
First Medical Department, Johannes Gutenberg University of Mainz, Mainz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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