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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
2009-4-3
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pubmed:abstractText |
The presence of orexins and their receptors in the gastrointestinal tract supports a local action of these peptides. Aim of the present study was to investigate the effects of orexin A (OXA) on the relaxant responses of the mouse gastric fundus. Mechanical responses of gastric strips were recorded via force-displacement transducers. The presence of orexin receptors (OX-1R) was also evaluated by immunocytochemistry. In carbachol precontracted strips and in the presence of guanethidine, electrical field stimulation (EFS) elicited a fast inhibitory response that may be followed, at the highest stimulation frequencies employed, by a sustained relaxation. All relaxant responses were abolished by TTX. The fast response was abolished by the nitric oxide (NO) synthesis inhibitor l-NNA (2x10(-4) M) as well as by the guanylate cyclase inhibitor ODQ (1x10(-6) M). OXA (3x10(-7) M) greatly increased the amplitude of the EFS-induced fast relaxation without affecting the sustained one. OXA also potentiated the amplitude of the relaxant responses elicited by the ganglionic stimulating agent DMPP (1x10(-5) M), but had no effects on the direct smooth muscle relaxant responses elicited by papaverine (1x10(-5) M) or VIP (1x10(-7) M). In the presence of l-NNA, the response to DMPP was reduced in amplitude and no longer influenced by OXA. The OX1 receptor antagonist SB-334867 (1x10(-5) M) reduced the amplitude of the EFS-induced fast relaxation without influencing neither the sustained responses nor those to papaverine and VIP. Immunocytochemistry showed the presence of neurons that co-express neuronal nitric oxide synthase and OX-1R. These results indicate that, in mouse gastric fundus, OXA exerts a modulatory action at the postganglionic level on the nitrergic neurotransmission.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(2-methylbenzoxazol-6-yl)-3-(1,5)n...,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoxazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Cholinergic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Dimethylphenylpiperazinium Iodide,
http://linkedlifedata.com/resource/pubmed/chemical/Guanethidine,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Oxytocin,
http://linkedlifedata.com/resource/pubmed/chemical/Papaverine,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Urea,
http://linkedlifedata.com/resource/pubmed/chemical/Vasoactive Intestinal Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/orexins,
http://linkedlifedata.com/resource/pubmed/chemical/oxytocin, Asp(5)-
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0167-0115
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
154
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
54-9
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pubmed:meshHeading |
pubmed-meshheading:19150469-Animals,
pubmed-meshheading:19150469-Benzoxazoles,
pubmed-meshheading:19150469-Carbachol,
pubmed-meshheading:19150469-Cholinergic Agonists,
pubmed-meshheading:19150469-Dimethylphenylpiperazinium Iodide,
pubmed-meshheading:19150469-Electric Stimulation,
pubmed-meshheading:19150469-Electrophysiology,
pubmed-meshheading:19150469-Female,
pubmed-meshheading:19150469-Gastric Fundus,
pubmed-meshheading:19150469-Guanethidine,
pubmed-meshheading:19150469-Guanylate Cyclase,
pubmed-meshheading:19150469-Immunohistochemistry,
pubmed-meshheading:19150469-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:19150469-Mice,
pubmed-meshheading:19150469-Mice, Inbred Strains,
pubmed-meshheading:19150469-Muscle, Smooth,
pubmed-meshheading:19150469-Muscle Contraction,
pubmed-meshheading:19150469-Muscle Relaxation,
pubmed-meshheading:19150469-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:19150469-Neuropeptides,
pubmed-meshheading:19150469-Nitric Oxide,
pubmed-meshheading:19150469-Oxytocin,
pubmed-meshheading:19150469-Papaverine,
pubmed-meshheading:19150469-Synaptic Transmission,
pubmed-meshheading:19150469-Tetrodotoxin,
pubmed-meshheading:19150469-Urea,
pubmed-meshheading:19150469-Vasoactive Intestinal Peptide
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pubmed:year |
2009
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pubmed:articleTitle |
Evidence for a modulatory role of orexin A on the nitrergic neurotransmission in the mouse gastric fundus.
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pubmed:affiliation |
Department of Physiological Sciences, University of Florence, Florence, Italy. mcaterina.baccari@unifi.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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