Linked Life Data

19150249

Source:http://linkedlifedata.com/resource/pubmed/id/19150249

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-2-9
pubmed:abstractText
Purified samples of CD45+ hematopoietic cells are a prerequisite for chimerism analysis in transplantation therapies, and are useful in various research and clinical settings such as functional and molecular analysis or disease diagnosis. Recently, we have established a flow-based adhesion molecule-dependent process for the purification of these cells from human bone marrow. However, for practical purposes, it is desirable to apply this approach to process small volumes of human blood. CD45+ cell purities were >94% when PBMNCs and plasma depleted blood were perfused through P-selectin coated microtubes. However, P-selectin surface failed to capture CD45+ cells when fresh blood prior to washing was perfused. The process requires a pre-step of plasma removal which otherwise inhibits interactions of cell surface PSGL-1 with immobilized P-selectin due to the presence of soluble PSGL-1 in plasma. We conclude that P-selectin can be used in a compact flow device to isolate and purify CD45+ cells directly from human peripheral blood. The process is simple, rapid, cost effective and represents a physiologic approach to the capture and purification of CD45+ MNCs from peripheral blood.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1096-0961
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
136-9
pubmed:meshHeading
pubmed:articleTitle
P-selectin-coated microtube for the purification of CD45+ hematopoietic cells directly from human peripheral blood.
pubmed:affiliation
Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies