Linked Life Data

19147118

Source:http://linkedlifedata.com/resource/pubmed/id/19147118

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-1-16
pubmed:abstractText
We report a method for efficient mutagenesis of DNA in large vectors without subcloning. Two segments of the target DNA sequence, one having a mutation introduced via a mutant primer, were amplified by PCR and then the purified fragments were ligated to a vector. The mutation efficiency was nearly 100%.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1347-4421
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
95-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer.
pubmed:affiliation
Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Suehiro-cho 1-7-29, Tsurumi-ku, Yokohama 230-0045, Japan.
pubmed:publicationType
Journal Article