|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2009-1-16
|
|
pubmed:abstractText |
This report describes the development and use of TnKnXSp, a selectable broad-host-range reporter transposon with a promoterless aphA gene. Insertion of TnKnXSp into the chromosome of a kanamycin-susceptible bacterium confers resistance to kanamycin only if aphA is transcribed from an active promoter adjacent to the insertion site. We designed TnKnXSp as a tool for identifying environmentally regulated promoters in bacteria and developed general methods for initial characterization of any TnKnXSp integrant. To identify putative iron-regulated promoters in Corynebacterium diphtheriae, we constructed TnKnXSp integrants and identified a subgroup that expressed kanamycin resistance under low-iron, but not high-iron, conditions. We characterized representative integrants with this phenotype, located the TnKnXSp insertion in each, and demonstrated that transcription of aphA was repressed under high-iron vs. low-iron growth conditions. We also demonstrated that TnKnXSp can be used in bacteria other than C. diphtheriae, including Escherichia coli and Bacillus subtilis. Our findings validate TnKnXSp as a useful tool for identifying environmentally regulated promoters in bacteria.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-10625401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-10972718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-11145416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-11386365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-11535792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-11985870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-12069964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-12169610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-12270831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-12450860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-13129217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-14602910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-14617647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-14622427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-15128537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-15447793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-15528561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-15629913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-16585757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-17275217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-1828057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-3041370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-8106325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-9317037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19146571-9353044
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
1574-6968
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
291
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
143-50
|
|
pubmed:dateRevised |
2011-9-26
|
|
pubmed:meshHeading |
pubmed-meshheading:19146571-Bacteria,
pubmed-meshheading:19146571-Bacterial Proteins,
pubmed-meshheading:19146571-DNA Transposable Elements,
pubmed-meshheading:19146571-DNA-Binding Proteins,
pubmed-meshheading:19146571-Genes, Reporter,
pubmed-meshheading:19146571-Genetic Vectors,
pubmed-meshheading:19146571-Iron,
pubmed-meshheading:19146571-Mutagenesis, Insertional,
pubmed-meshheading:19146571-Promoter Regions, Genetic,
pubmed-meshheading:19146571-Protein Binding,
pubmed-meshheading:19146571-Transcription, Genetic
|
|
pubmed:year |
2009
|
|
pubmed:articleTitle |
Development and use of a selectable, broad-host-range reporter transposon for identifying environmentally regulated promoters in bacteria.
|
|
pubmed:affiliation |
Department of Microbiology, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
