Source:http://linkedlifedata.com/resource/pubmed/id/19142948
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2009-1-21
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| pubmed:abstractText |
Hepatic ischemia/reperfusion (I/R) injury is an inevitable consequence during liver surgery. Ischemic preconditioning (IPC) has been shown to protect the livers from I/R injury, partially mediated by preservation of hepatic ATP contents. However, the precise molecular mechanisms of these events remain poorly elucidated. In this study, liver proteomes of the mice subjected to I/R injury pretreated with or without IPC were analyzed using 2-DE combined with MALDI-TOF/TOF mass analysis. Twenty proteins showing more than 1.5-fold difference were identified in the livers upon I/R injury. Among these proteins, four proteins were further regulated by IPC when compared with nonpretreated controls. One of these proteins, ATP synthase beta subunit (ATP5beta) catalyzes the rate-limiting step of ATP formation. The expression level of ATP5beta, which was further validated by Western blot analysis, was significantly decreased upon I/R injury while turned over by IPC pretreatment. Change pattern of hepatic ATP corresponded with that of ATP5beta expression, indicating that increasing hepatic ATP5beta expression might be a reason for ATP-preserving effect of IPC. In summary, this study provided new clues for understanding the mechanisms of IPC against I/R injury. The protective role of ATP5beta might give evidences for developing new therapeutic approaches against hepatic I/R injury.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Alanine Transaminase,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartate Aminotransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Proton-Translocating...,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
|
| pubmed:issn |
1615-9861
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
9
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
409-19
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| pubmed:meshHeading |
pubmed-meshheading:19142948-Adenosine Triphosphate,
pubmed-meshheading:19142948-Alanine Transaminase,
pubmed-meshheading:19142948-Amino Acid Sequence,
pubmed-meshheading:19142948-Analysis of Variance,
pubmed-meshheading:19142948-Animals,
pubmed-meshheading:19142948-Aspartate Aminotransferases,
pubmed-meshheading:19142948-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:19142948-Gene Expression Regulation,
pubmed-meshheading:19142948-Ischemic Preconditioning,
pubmed-meshheading:19142948-Liver,
pubmed-meshheading:19142948-Liver Diseases,
pubmed-meshheading:19142948-Male,
pubmed-meshheading:19142948-Mice,
pubmed-meshheading:19142948-Mice, Inbred C57BL,
pubmed-meshheading:19142948-Mitochondrial Proton-Translocating ATPases,
pubmed-meshheading:19142948-Molecular Sequence Data,
pubmed-meshheading:19142948-Proteins,
pubmed-meshheading:19142948-Proteomics,
pubmed-meshheading:19142948-Reperfusion Injury
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| pubmed:year |
2009
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| pubmed:articleTitle |
Proteomic analysis of hepatic ischemia/reperfusion injury and ischemic preconditioning in mice revealed the protective role of ATP5beta.
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| pubmed:affiliation |
Department of Gastroenterology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, PR China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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