Linked Life Data

19141686

Source:http://linkedlifedata.com/resource/pubmed/id/19141686

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-3-25
pubmed:abstractText
17beta-estradiol (E2) exerts inhibitory actions on the nitric oxide pathway in rat adult pituitary glands. Previously, we reported that in vivo E2 acute treatment had opposite effects on soluble guanylyl cyclase (sGC) subunits, increasing alpha1- and decreasing beta1-subunit protein and mRNA expression and decreasing sGC activity in immature rats. Here we studied the E2 effect on sGC protein and mRNA expression in anterior pituitary gland from adult female rats to address whether the maturation of the hypothalamus-pituitary axis influences its effects and to corroborate whether these effects occur in physiological conditions such as during estrous cycle. E2 administration causes the same effect on sGC as seen in immature rats, and these effects are estrogen receptor dependent. These results suggest that E2 is the main effector of these changes. Since the sGC alpha-subunit increases while the sGC activity decreases, we studied if other less active isoforms of the sGC alpha-subunit are expressed. Here we show for the first time that sGCalpha2 and sGCalpha2 inhibitory (alpha2i) isoforms are expressed in this gland, but only sGCalpha2i mRNA increased after E2 acute treatment. Finally, to test whether E2 effects take place under a physiological condition, sGC subunit expression was monitored over estrous cycle. sGCalpha1, -beta1, and -alpha2i fluctuate along estrous cycle, and these changes are directly related with E2 level fluctuations rather than to NO level variations. These findings show that E2 physiologically regulates sGC expression and highlight a novel mechanism by which E2 downregulates sGC activity in rat anterior pituitary gland.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0193-1849
pubmed:author
pubmed:issnType
Print
pubmed:volume
296
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E731-7
pubmed:meshHeading
pubmed-meshheading:19141686-Animals, pubmed-meshheading:19141686-Cells, Cultured, pubmed-meshheading:19141686-Cyclic GMP, pubmed-meshheading:19141686-Enzyme Activation, pubmed-meshheading:19141686-Estradiol, pubmed-meshheading:19141686-Estrogen Antagonists, pubmed-meshheading:19141686-Estrous Cycle, pubmed-meshheading:19141686-Female, pubmed-meshheading:19141686-Gene Expression Regulation, Enzymologic, pubmed-meshheading:19141686-Guanylate Cyclase, pubmed-meshheading:19141686-Nitric Oxide, pubmed-meshheading:19141686-Pituitary Gland, Anterior, pubmed-meshheading:19141686-Protein Subunits, pubmed-meshheading:19141686-RNA, Messenger, pubmed-meshheading:19141686-Rats, pubmed-meshheading:19141686-Rats, Wistar, pubmed-meshheading:19141686-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:19141686-Receptors, Estrogen
pubmed:year
2009
pubmed:articleTitle
Nitric oxide sensitive-guanylyl cyclase subunit expression changes during estrous cycle in anterior pituitary glands.
pubmed:affiliation
Departamento de Química Biológica, IQUIFIB, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't