|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2009-1-14
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The ROP2 protein and its paralogs are important virulence factors secreted into the host cell by the parasite Toxoplasma gondii. Here we describe the crystal structure of a large and soluble domain of mature ROP2, representative of the ROP2-like protein family. This is a structure of a protein-kinase fold that is devoid of catalytic residues and does not bind ATP. Various structural extensions constitute a signature of this protein family and act to maintain the protein kinase in an open conformation. Our ROP2 structure rules out a previous structural model of attachment of ROP2-like proteins to the parasitophorous vacuole membrane. We propose an alternative mode of membrane attachment implicating basic and amphiphatic helices present in the flexible N terminus of ROP2.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0969-2126
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
14
|
|
pubmed:volume |
17
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
139-46
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:19141290-Amino Acid Sequence,
pubmed-meshheading:19141290-Animals,
pubmed-meshheading:19141290-Base Sequence,
pubmed-meshheading:19141290-Binding Sites,
pubmed-meshheading:19141290-Cell Line,
pubmed-meshheading:19141290-Circular Dichroism,
pubmed-meshheading:19141290-Cricetinae,
pubmed-meshheading:19141290-DNA Primers,
pubmed-meshheading:19141290-Membrane Proteins,
pubmed-meshheading:19141290-Models, Molecular,
pubmed-meshheading:19141290-Molecular Sequence Data,
pubmed-meshheading:19141290-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:19141290-Peptides,
pubmed-meshheading:19141290-Protein Folding,
pubmed-meshheading:19141290-Protein Kinases,
pubmed-meshheading:19141290-Protozoan Proteins,
pubmed-meshheading:19141290-Sequence Homology, Amino Acid,
pubmed-meshheading:19141290-Toxoplasma,
pubmed-meshheading:19141290-Virulence
|
|
pubmed:year |
2009
|
|
pubmed:articleTitle |
ROP2 from Toxoplasma gondii: a virulence factor with a protein-kinase fold and no enzymatic activity.
|
|
pubmed:affiliation |
Atelier de Bio- et Chimie Informatique Structurale, Centre de Biochimie Structurale, CNRS, UMR5048, Universités Montpellier 1 et 2, F34090 Montpellier, France. labesse@cbs.cnrs.fr
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
