Linked Life Data

19138067

Source:http://linkedlifedata.com/resource/pubmed/id/19138067

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-1-28
pubmed:abstractText
The nucleosome core particle is the fundamental unit of chromatin structure and at its heart is the histone core octamer composed of histones H4, H3, H2A and H2B. To understand the structure dynamics and function of chromatin it is important to be able to probe the structures of its component parts in a variety of ways. Site directed spin-labeling technology has enabled the insertion of nitroxide spin labels at positions on the surface of the H3 histones and these have been assembled into histone octamers. Pulsed EPR, and in particular the PELDOR or DEER experiments have been performed and provided extremely well defined dipolar oscillations, over long time periods. From the PELDOR data we have been able to derive distance distributions of between 60 and 70 A. The distances measured, are among the longest well-defined PELDOR measurements on a biological system to date, spanning the width of the histone core particle and approaching what has been often defined as the limit of distance measurement by this technique. Relatively minor differences to the crystal structures have been observed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1520-5126
pubmed:author
pubmed:issnType
Electronic
pubmed:day
4
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1348-9
pubmed:dateRevised
2010-4-20
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Long distance PELDOR measurements on the histone core particle.
pubmed:affiliation
School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't