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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-2-4
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pubmed:abstractText |
The elucidation of a code for regulated splicing has been a long-standing goal in understanding the control of post-transcriptional gene expression events that are crucial for cell survival, differentiation and development. We decoded functional RNA elements in vivo by constructing an RNA map for the cell type-specific splicing regulator FOX2 (also known as RBM9) via cross-linking immunoprecipitation coupled with high-throughput sequencing (CLIP-seq) in human embryonic stem cells. The map identified a large cohort of specific FOX2 targets, many of which are themselves splicing regulators, and comparison between the FOX2 binding profile and validated splicing events revealed a general rule for FOX2-regulated exon inclusion or skipping in a position-dependent manner. These findings suggest that FOX2 functions as a critical regulator of a splicing network, and we further show that FOX2 is important for the survival of human embryonic stem cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-11121071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-11376152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-12114529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-12574126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-12626338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-12840041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-14615540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-15708978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-15790528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-15824060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-15905405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-16024819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-16136043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-16260614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-16362037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-16449636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-16537540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-16793546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-16839192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-17065982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-17530930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-17558387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-17606642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-17679093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-18043867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-18794351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-4128323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-49354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-7561136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19136955-9804556
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1545-9985
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
130-7
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pubmed:dateRevised |
2011-10-10
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pubmed:meshHeading |
pubmed-meshheading:19136955-Alternative Splicing,
pubmed-meshheading:19136955-Cell Survival,
pubmed-meshheading:19136955-Embryonic Stem Cells,
pubmed-meshheading:19136955-Exons,
pubmed-meshheading:19136955-Humans,
pubmed-meshheading:19136955-Immunoprecipitation,
pubmed-meshheading:19136955-RNA Splice Sites,
pubmed-meshheading:19136955-RNA-Binding Proteins,
pubmed-meshheading:19136955-Repressor Proteins,
pubmed-meshheading:19136955-Sequence Analysis, RNA
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pubmed:year |
2009
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pubmed:articleTitle |
An RNA code for the FOX2 splicing regulator revealed by mapping RNA-protein interactions in stem cells.
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pubmed:affiliation |
Crick-Jacobs Center for Theoretical and Computational Biology, Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037, USA. geneyeo@ucsd.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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