Linked Life Data

1913638

Source:http://linkedlifedata.com/resource/pubmed/id/1913638

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1991-10-29
pubmed:abstractText
We devised a new in vitro method to estimate the proportion of dividing cells and the potential doubling time (Tpot) of tumors using the same technique as with the cytokinesis-block micronucleus assay. The usefulness of this methodology was confirmed by comparing the data with those obtained by flow cytometry after bromodeoxyuridine (BrdUrd) incorporation. Xenografted human and murine tumors were excised 0.5-8 h after BrdUrd injection and disaggregated to single cells. A portion of these cells was then plated in dishes to which 1 or 2 micrograms/ml cytochalasin B were added. These concentrations of cytochalasin B blocked cytokinesis but not karyokinesis with the result that cells became multinucleate after mitoses. At every 12 or 24 h of culture, the proportion of multinucleate cells and the total number of nuclei and cells were scored. The remaining cells were analyzed with a flow cytometer and the BrdUrd-labeling index and Tpot were determined. In all 8 tumor lines studied, the proportion of multinucleate cells reached a plateau within 3-7 days of culture, and we therefore defined the dividing fraction as the plateau value. The dividing fraction ranged between 33 and 98% and clearly tended to be high in rapidly growing tumors. A significant correlation was seen between the dividing fraction and BrdUrd-labeling index (r = 0.74, P less than 0.001). The increase in the average number of nuclei per cell also tended to be higher in rapidly growing tumors. The Tpot was estimated as the time for this nucleus/cell ratio to reach 2.0. In 7 of 8 tumor lines, Tpot values estimated by this method compared reasonably with those estimated by the BrdUrd method. Therefore, this simple technique, originally developed for radiosensitivity prediction, would also seem to be useful in estimating tumor proliferative activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5134-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Estimation of the dividing fraction and potential doubling time of tumors using cytochalasin B.
pubmed:affiliation
Institute of Medical Radiobiology, University of Essen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't