| pubmed-article:19135102 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19135102 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:19135102 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19135102 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:19135102 | lifeskim:mentions | umls-concept:C0599772 | lld:lifeskim |
| pubmed-article:19135102 | lifeskim:mentions | umls-concept:C0079411 | lld:lifeskim |
| pubmed-article:19135102 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:19135102 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:19135102 | pubmed:dateCreated | 2009-4-20 | lld:pubmed |
| pubmed-article:19135102 | pubmed:abstractText | Conditional gene knockout by homologous recombination combined with an inducible gene expression system is a powerful approach for studying gene function, although homologous recombination in human cells occurs infrequently. The tetracycline-regulated gene expression (Tet-Off) system is a convenient method for achieving conditional gene knockout, but it is not always promising in Nalm-6, a rare human cell line highly effective for gene targeting. Here we modified the Tet-Off system and applied it to the Nalm-6 cell line successfully by using an internal ribosome entry site to drive a selectable marker from the same tetracycline-responsive promoter for the transgene. We also inserted the gene for the tetracycline-controlled transactivator under the control of a potent CAG promoter. These modifications enabled us to easily obtain rare clones that express optimal amounts of tetracycline-regulated transgenes. We thereby generated a 'tetracycline-inducible conditional gene knockout' for the proliferation-associated SNF2-like gene (PASG) in a Nalm-6 cell line, in which the expression of PASG can be depleted in a tetracycline-dependent manner on a knockout background. This method is applicable to any human genes, making this gene-targeting system using the Nalm-6 cell line a promising tool for analyzing gene function. | lld:pubmed |
| pubmed-article:19135102 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19135102 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19135102 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19135102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19135102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19135102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19135102 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19135102 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19135102 | pubmed:issn | 1873-4863 | lld:pubmed |
| pubmed-article:19135102 | pubmed:author | pubmed-author:KoyamaHidekiH | lld:pubmed |
| pubmed-article:19135102 | pubmed:author | pubmed-author:AdachiNoritak... | lld:pubmed |
| pubmed-article:19135102 | pubmed:author | pubmed-author:NishijimaHito... | lld:pubmed |
| pubmed-article:19135102 | pubmed:author | pubmed-author:IiizumiSusumu... | lld:pubmed |
| pubmed-article:19135102 | pubmed:author | pubmed-author:OnoTatsuyaT | lld:pubmed |
| pubmed-article:19135102 | pubmed:author | pubmed-author:ShibaharaKei-... | lld:pubmed |
| pubmed-article:19135102 | pubmed:author | pubmed-author:MorohoshiAkan... | lld:pubmed |
| pubmed-article:19135102 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19135102 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:19135102 | pubmed:volume | 141 | lld:pubmed |
| pubmed-article:19135102 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19135102 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19135102 | pubmed:pagination | 1-7 | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:meshHeading | pubmed-meshheading:19135102... | lld:pubmed |
| pubmed-article:19135102 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19135102 | pubmed:articleTitle | Generation of tetracycline-inducible conditional gene knockout cells in a human Nalm-6 cell line. | lld:pubmed |
| pubmed-article:19135102 | pubmed:affiliation | Department of Integrated Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan. | lld:pubmed |
| pubmed-article:19135102 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19135102 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
