Linked Life Data

19135038

Source:http://linkedlifedata.com/resource/pubmed/id/19135038

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2009-3-24
pubmed:abstractText
Chronic inflammation, a process linked to increased oxidative stress, may induce many diseases. Whether beta-carotene prevents inflammation is unclear. Using phorbol-12-myristate-13-acetate (PMA)-stimulated HL-60 cells, we investigated the effects of 2 or 20 microM beta-carotene on the inflammatory reaction of monocyte/macrophage-like cells and the modulation of 20 microM quercetin or naringenin, two flavonoids, of the effects of beta-carotene. The effects of quercetin and naringenin were compared with that of alpha-tocopherol, a well-known antioxidant. The stimulated HL-60 cells were also co-incubated with A549 cells to investigate the DNA-damaging ability of the stimulated monocyte/macrophage-like cells on target cells. Our results showed that preincubation with 20 microM beta-carotene significantly enhanced the release of two pro-inflammatory mediators, interleukin-8 and tumor necrosis factor-alpha, in PMA-stimulated HL-60 cells and slightly increased the DNA-damaging ability of these cells. By contrast, 2 microM beta-carotene had an inhibitory effect on the inflammatory reaction in PMA-stimulated cells. The higher dose of beta-carotene also exerted pro-inflammatory effects in lipopolysaccharide-stimulated RAW264.7 cells. Furthermore, quercetin, naringenin, and alpha-tocopherol partly suppressed the pro-inflammatory effects of 20 microM beta-carotene on PMA-stimulated HL-60 cells, and the suppressing effects of quercetin and naringenin were better than or similar to those of alpha-tocopherol. Quercetin also additively or synergistically enhanced the inhibitory effects of 2 microM beta-carotene on the secretion of pro-inflammatory mediators and the DNA-damaging ability of PMA-stimulated HL-60 cells. The mechanisms underlying the effect of the flavonoids were associated with their antioxidant activity and inhibition of the production of pro-inflammatory cytokines. Our results urge consideration of the safety of beta-carotene supplementation concerning effects on inflammation and suggest that the interaction between beta-carotene and quercetin or naringenin may alter the effects of beta-carotene on the secretion of pro-inflammatory mediators.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1872-7786
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
179
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
386-93
pubmed:meshHeading
pubmed-meshheading:19135038-Animals, pubmed-meshheading:19135038-Cell Differentiation, pubmed-meshheading:19135038-Cells, Cultured, pubmed-meshheading:19135038-DNA Damage, pubmed-meshheading:19135038-Dose-Response Relationship, Drug, pubmed-meshheading:19135038-Flavanones, pubmed-meshheading:19135038-HL-60 Cells, pubmed-meshheading:19135038-Humans, pubmed-meshheading:19135038-Interleukin-8, pubmed-meshheading:19135038-Lipopolysaccharides, pubmed-meshheading:19135038-Mice, pubmed-meshheading:19135038-Quercetin, pubmed-meshheading:19135038-Reactive Oxygen Species, pubmed-meshheading:19135038-Tetradecanoylphorbol Acetate, pubmed-meshheading:19135038-Thiobarbituric Acid Reactive Substances, pubmed-meshheading:19135038-Time Factors, pubmed-meshheading:19135038-Tumor Necrosis Factor-alpha, pubmed-meshheading:19135038-alpha-Tocopherol, pubmed-meshheading:19135038-beta Carotene
pubmed:year
2009
pubmed:articleTitle
Interactions of beta-carotene and flavonoids on the secretion of pro-inflammatory mediators in an in vitro system.
pubmed:affiliation
Institute of Nutritional Science, Chung Shan Medical University, Taichung, Taiwan, ROC. suzyyeh@csmu.edu.tw
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't