Linked Life Data

19133651

Source:http://linkedlifedata.com/resource/pubmed/id/19133651

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-4-6
pubmed:abstractText
MicroRNAs (miRNAs) have recently been proposed as a versatile class of molecules involved in regulation of various biological processes. Although there is emerging evidence that some microRNAs can function as oncogenes or tumor suppressors, the specific role of miRNA in human hepatocellular carcinoma (HCC) is unclear at this point. In this study, we examined the microRNA expression profiles in a set of 20 human HCC specimens by miRNA microarray and quantitative real-time polymerase chain reaction. The results showed that among the 20 HCC samples analyzed, microRNA-101 was significantly down-regulated twofold or more (twofold to 20-fold) in 16 samples compared with the matching nontumoral liver tissues. Using both a luciferase reporter assay and Western blot analysis, we showed that microRNA-101 repressed the expression of v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS) oncogene, a key component of the activator protein-1 (AP-1) transcription factor. Moreover, using a luciferase expression vector (pAP-1-Luc) driven by seven copies of an AP-1 cis-element, we observed that microRNA-101 expression inhibited phorbol 12-myristate 13-acetate (PMA)-induced AP-1 activity. In in vitro Matrigel invasion and Transwell migration assays, enhanced microRNA-101 expression inhibited the invasion and migration of cultured HCC cells, respectively. These findings suggest that microRNA-101 may play an important role in HCC. CONCLUSION: MicroRNA-101, which is aberrantly expressed in HCC, could repress the expression of the FOS oncogene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1527-3350
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1194-202
pubmed:meshHeading
pubmed-meshheading:19133651-3' Untranslated Regions, pubmed-meshheading:19133651-Adult, pubmed-meshheading:19133651-Aged, pubmed-meshheading:19133651-Blotting, Western, pubmed-meshheading:19133651-Carcinoma, Hepatocellular, pubmed-meshheading:19133651-Cell Line, Tumor, pubmed-meshheading:19133651-Cell Movement, pubmed-meshheading:19133651-Female, pubmed-meshheading:19133651-Gene Expression Profiling, pubmed-meshheading:19133651-Gene Expression Regulation, Neoplastic, pubmed-meshheading:19133651-Genes, Reporter, pubmed-meshheading:19133651-Genes, fos, pubmed-meshheading:19133651-Humans, pubmed-meshheading:19133651-Liver Neoplasms, pubmed-meshheading:19133651-Male, pubmed-meshheading:19133651-MicroRNAs, pubmed-meshheading:19133651-Middle Aged, pubmed-meshheading:19133651-Proto-Oncogene Proteins c-fos, pubmed-meshheading:19133651-RNA, Messenger, pubmed-meshheading:19133651-Sequence Analysis, DNA, pubmed-meshheading:19133651-Tetradecanoylphorbol Acetate, pubmed-meshheading:19133651-Transcription Factor AP-1
pubmed:year
2009
pubmed:articleTitle
MicroRNA-101 regulates expression of the v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS) oncogene in human hepatocellular carcinoma.
pubmed:affiliation
Beijing Institute of Radiation Medicine, Beijing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't