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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2009-1-9
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pubmed:abstractText |
Hantaan virus (HTNV) is an Old World hantavirus associated with hemorrhagic fever with renal syndrome (HFRS). To visualize the localization of the L protein of HTNV strain 84FLi within cells, a fusion protein composed of enhanced green fluorescent protein and L protein, EGFP-L, was expressed in Vero cells. The 273 KDa expressed fusion protein of EGFP-L localized in the perinuclear region. We also described the development of a reverse genetics system for HTNV strain 84FLi. The RNA polymerase I (pol I)-mediated transcription system was used to generate artificial viral RNA genome segments (minigenomes), which contained the chloramphenicol acetyltransferase (CAT) reporter gene in antisense (virus RNA) or sense (virus-complementary RNA) orientation flanked by the noncoding regions of HTNV 84FLi L segment. CAT could be detected in cells after transfection, indicating the successful encapsidation, transcription and replication of the pol I-derived minigenomes. The passaged transfer of CAT demonstrates that recombinant virus containing packaged pol I-derived minigenomes has been produced. This system may be helpful in studying the gene function and pathogenesis of HTNV.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/L protein, hantavirus,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Polymerase I,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Replicase,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/enhanced green fluorescent protein
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1600-0463
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1089-96
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pubmed:meshHeading |
pubmed-meshheading:19133012-Animals,
pubmed-meshheading:19133012-Cercopithecus aethiops,
pubmed-meshheading:19133012-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:19133012-Cytoplasm,
pubmed-meshheading:19133012-Gene Expression,
pubmed-meshheading:19133012-Genes, Reporter,
pubmed-meshheading:19133012-Genome, Viral,
pubmed-meshheading:19133012-Green Fluorescent Proteins,
pubmed-meshheading:19133012-Hantaan virus,
pubmed-meshheading:19133012-Hantavirus Infections,
pubmed-meshheading:19133012-Humans,
pubmed-meshheading:19133012-RNA, Viral,
pubmed-meshheading:19133012-RNA Polymerase I,
pubmed-meshheading:19133012-RNA Replicase,
pubmed-meshheading:19133012-Recombinant Fusion Proteins,
pubmed-meshheading:19133012-Reverse Transcription,
pubmed-meshheading:19133012-Vero Cells,
pubmed-meshheading:19133012-Viral Proteins
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pubmed:year |
2008
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pubmed:articleTitle |
Expression of L protein of Hantaan virus 84FLi strain and its application for recovery of minigenomes.
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pubmed:affiliation |
Center of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, P. R. China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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