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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2009-2-9
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pubmed:abstractText |
Two isozymes for human acyl-coenzyme A:diacylglycerol acyltransferase (DGAT), DGAT1 and DGAT2, were independently expressed in DGAT-deficient Saccharomyces cerevisiae to establish DGAT1- and DGAT2-S. cerevisiae. The selectivity of DGAT inhibitors of natural origin towards the isozymes was assessed in enzyme assays using the microsomal fractions prepared from DGAT1- and DGAT2-S. cerevisiae. Amidepsines and xanthohumol inhibited DGAT1 and DGAT2 with similar potency, whereas roselipins were found to inhibit DGAT2 selectively.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-8820
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
51-4
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pubmed:meshHeading |
pubmed-meshheading:19132064-Animals,
pubmed-meshheading:19132064-DNA, Complementary,
pubmed-meshheading:19132064-Diacylglycerol O-Acyltransferase,
pubmed-meshheading:19132064-Enzyme Inhibitors,
pubmed-meshheading:19132064-Humans,
pubmed-meshheading:19132064-Isoenzymes,
pubmed-meshheading:19132064-Liver,
pubmed-meshheading:19132064-Molecular Sequence Data,
pubmed-meshheading:19132064-Plasmids,
pubmed-meshheading:19132064-Rats,
pubmed-meshheading:19132064-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19132064-Saccharomyces cerevisiae
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pubmed:year |
2009
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pubmed:articleTitle |
Expression of two human acyl-CoA:diacylglycerol acyltransferase isozymes in yeast and selectivity of microbial inhibitors toward the isozymes.
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pubmed:affiliation |
School of Pharmacy, Kitasato University, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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