Linked Life Data

19131114

Source:http://linkedlifedata.com/resource/pubmed/id/19131114

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-3-9
pubmed:databankReference
pubmed:abstractText
Toll like receptors (TLRs) are a class of pattern recognition receptors belonging to the innate immune system. Mutations in the protein coding region of TLRs are associated with altered responsiveness to pathogen-associated molecular patterns (PAMPs). A search was performed for novel mutations in bovine TLR1, TLR2 and TLR4 genes associated with the Mycobacterium avium subsp. paratuberculosis (MAP) infection. The work was also focused on the assessment of linkage between well known mutations in TLR genes (TLR2: Arg677Trp, Pro681His and Arg753Gln; TLR4: Asp299Gly and Thr399Ile), and the susceptibility of cattle to MAP infection. Detection of MAP infection in cattle population (n=711) was based on IS900 PCR, which revealed 22.50% (n=160) MAP positivity. Known mutations in TLR2 and TLR4 genes were not found in cattle population. A novel mutation Val220Met was associated (Odd's ratio, OR-3.459) with increased susceptibility to MAP infection. Toll/interleukin-1 receptor (TIR) domain of TLR2 was screened for the presence of mutations, wherein a novel Ile680Val mutation was linked with MAP infection. In silico analysis of the bovine TLR4 ectodomain (ECD) revealed the polymorphic nature of the central ECD and irregularities in the central LRR motifs. LRR11 of the TLR4 showed five missense mutations possibly linked with the increased susceptibility to MAP infection. The most critical position that may alter the pathogen recognition of TLR molecule was 4th residue downstream to LRR domain. Two such missense mutations in TLR4 (Asp299Asn downstream to LRR11, and Gly389Ser downstream to LRR15) were associated with MAP infection. Briefly, the work describes novel mutations in the bovine TLRs and presents their association with the MAP infection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0165-2427
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
381-8
pubmed:meshHeading
pubmed-meshheading:19131114-Amino Acid Motifs, pubmed-meshheading:19131114-Amino Acid Sequence, pubmed-meshheading:19131114-Animals, pubmed-meshheading:19131114-Base Sequence, pubmed-meshheading:19131114-Cattle, pubmed-meshheading:19131114-Cattle Diseases, pubmed-meshheading:19131114-DNA, pubmed-meshheading:19131114-Female, pubmed-meshheading:19131114-Genetic Predisposition to Disease, pubmed-meshheading:19131114-Genetic Variation, pubmed-meshheading:19131114-Molecular Sequence Data, pubmed-meshheading:19131114-Mutation, pubmed-meshheading:19131114-Mutation, Missense, pubmed-meshheading:19131114-Mycobacterium avium subsp. paratuberculosis, pubmed-meshheading:19131114-Paratuberculosis, pubmed-meshheading:19131114-Point Mutation, pubmed-meshheading:19131114-Polymerase Chain Reaction, pubmed-meshheading:19131114-Polymorphism, Single Nucleotide, pubmed-meshheading:19131114-Prevalence, pubmed-meshheading:19131114-Sequence Alignment, pubmed-meshheading:19131114-Slovakia, pubmed-meshheading:19131114-Toll-Like Receptors
pubmed:year
2009
pubmed:articleTitle
Toll-like receptors TLR1, TLR2 and TLR4 gene mutations and natural resistance to Mycobacterium avium subsp. paratuberculosis infection in cattle.
pubmed:affiliation
Laboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine, Kosice, Slovakia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't