Linked Life Data

19130676

Source:http://linkedlifedata.com/resource/pubmed/id/19130676

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-1-8
pubmed:abstractText
A strain of the yeast Saccharomyces cerevisiae coding for KHR on the chromosome secreted a toxin that kills sensitive yeasts. The transformants of multicopy vectors carrying the KHR gene could secrete 3-4-fold the killer toxin of the donor strain. This toxic substance was purified 80-fold in specific activity from the culture filtrate by gel filtration and hydrophobic column chromatography. The purified toxin gave a single protein band with molecular mass of 20 kDa on SDS-PAGE and had an isoelectric point of pH 5.3. The toxin had novel killer activity against Candida glabrata and S. cerevisiae, but did not affect bacteria, fungi, or other yeasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0002-1369
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
505-9
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Isolation and properties of a chromosome-dependent KHR killer toxin in Saccharomyces cerevisiae.
pubmed:affiliation
National Research Institute of Brewing, 2-6-30 Takinogawa, Kita-ku, Tokyo 114, Japan.
pubmed:publicationType
Journal Article, Comparative Study