Linked Life Data

19129462

Source:http://linkedlifedata.com/resource/pubmed/id/19129462

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-3-5
pubmed:abstractText
In the adult skeletal muscle, various kinds of trauma promote proliferation of satellite cells that differentiate into myoblasts forming new myofibers or to repair the damaged one. The aim of present work was to perform a comparative spatial and temporal analysis of connexin (Cx) 37, Cx39, Cx40, Cx43, and Cx45 expression in the adult regenerating skeletal muscle in response to crush injury. Within 24 h from injury, Cx37 expression was upregulated in the endothelial cells of blood vessels, and, 5 days after injury, Cx37-expressing cells were found inside the area of lesion and formed clusters generating new blood vessels with endothelial cells expressing Cx37. Three days after injury, Cx39 mRNA was selectively expressed in myogenin-positive cells, forming rows of closely apposed cell nuclei fusing in myotubes. Cx40 mRNA-labeled cells were observed within 24 h from injury in the endothelium of blood vessels, and, 5 days after lesion, Cx40-labeled cells were found inside the area of lesion-forming rows of myogenin-positive, closely apposed cells coexpressing Cx39. Within 24 h from lesion, both Cx43 and Cx45 mRNAs were upregulated in individual cells, and some of them were positive for M-cadherin. Three days after injury, a large number of both Cx43 and Cx45 mRNA-labeled and myogenin-positive cells were found inside the area of lesion. Taken together, these results show that at least four Cxs, out of five expressed in regenerating skeletal muscle, can be differentially involved in communication of myogenic cells during the process of cell proliferation, aggregation, and fusion to form new myotubes or to repair damaged myofibers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0363-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
296
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C593-606
pubmed:dateRevised
2010-5-26
pubmed:meshHeading
pubmed-meshheading:19129462-Animals, pubmed-meshheading:19129462-Cell Aggregation, pubmed-meshheading:19129462-Cell Fusion, pubmed-meshheading:19129462-Cell Proliferation, pubmed-meshheading:19129462-Connexin 43, pubmed-meshheading:19129462-Connexins, pubmed-meshheading:19129462-Constriction, pubmed-meshheading:19129462-Endothelial Cells, pubmed-meshheading:19129462-Gene Expression Regulation, pubmed-meshheading:19129462-Immunohistochemistry, pubmed-meshheading:19129462-In Situ Hybridization, pubmed-meshheading:19129462-Male, pubmed-meshheading:19129462-Muscle, Skeletal, pubmed-meshheading:19129462-Muscle Fibers, Skeletal, pubmed-meshheading:19129462-Neovascularization, Physiologic, pubmed-meshheading:19129462-RNA, Messenger, pubmed-meshheading:19129462-Rats, pubmed-meshheading:19129462-Rats, Wistar, pubmed-meshheading:19129462-Regeneration, pubmed-meshheading:19129462-Time Factors
pubmed:year
2009
pubmed:articleTitle
Regulation of connexin gene expression during skeletal muscle regeneration in the adult rat.
pubmed:affiliation
Dept. of Experimental Medicine, Section of Human Physiology, Univ. of Palermo, corso Tukory 129, 90134 Palermo, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't