| pubmed-article:19127523 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19127523 | lifeskim:mentions | umls-concept:C1185739 | lld:lifeskim |
| pubmed-article:19127523 | lifeskim:mentions | umls-concept:C0026609 | lld:lifeskim |
| pubmed-article:19127523 | lifeskim:mentions | umls-concept:C0521329 | lld:lifeskim |
| pubmed-article:19127523 | lifeskim:mentions | umls-concept:C0598964 | lld:lifeskim |
| pubmed-article:19127523 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:19127523 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:19127523 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:19127523 | lifeskim:mentions | umls-concept:C0205088 | lld:lifeskim |
| pubmed-article:19127523 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19127523 | pubmed:dateCreated | 2009-2-2 | lld:pubmed |
| pubmed-article:19127523 | pubmed:abstractText | Molecular studies have revealed the presence of R-type voltage-gated Ca(2+) channels at pre- and postsynaptic regions; however, no evidence for the participation of these channels in transmitter release has been presented for the spinal cord. Here we characterize the effects of SNX-482, a selective R channel blocker, on the monosynaptic excitatory postsynaptic potentials (EPSPs) evoked in motoneurons by stimulation of dorsolateral funiculus (DLF) terminals in a slice preparation from the adult turtle spinal cord. SNX-482 inhibited neurotransmission in a dose-dependent manner, with an IC(50) of approximately 9 +/- 1 nM. The EPSP time course and membrane time constant of the motoneurons were not altered, suggesting a presynaptic mechanism. The toxin inhibited the residual component of the EPSPs recorded in the presence of N- and P/Q-type Ca(2+) channel blockers, strongly suggesting a role for the R channels in neurotransmission at the spinal cord DLF terminals. Consistently with this, RT-PCR analysis of turtle spinal cord segments revealed the expression of the Ca(V)2.3 pore-forming (alpha(1E)) subunit of R channels, whereas the use of anti-alpha(1E)-specific antibodies resulted in its localization in the DLF fibers as demonstrated by immunohistochemistry coupled with laser confocal microscopy. | lld:pubmed |
| pubmed-article:19127523 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19127523 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19127523 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19127523 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19127523 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19127523 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19127523 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19127523 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19127523 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:19127523 | pubmed:issn | 1096-9861 | lld:pubmed |
| pubmed-article:19127523 | pubmed:author | pubmed-author:FelixRicardoR | lld:pubmed |
| pubmed-article:19127523 | pubmed:author | pubmed-author:VergaraPaulaP | lld:pubmed |
| pubmed-article:19127523 | pubmed:author | pubmed-author:Delgado-Lezam... | lld:pubmed |
| pubmed-article:19127523 | pubmed:author | pubmed-author:CastroAlberto... | lld:pubmed |
| pubmed-article:19127523 | pubmed:author | pubmed-author:AguilarJustoJ | lld:pubmed |
| pubmed-article:19127523 | pubmed:author | pubmed-author:SegoviaJoseJ | lld:pubmed |
| pubmed-article:19127523 | pubmed:author | pubmed-author:AndradeArturo... | lld:pubmed |
| pubmed-article:19127523 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19127523 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:19127523 | pubmed:volume | 513 | lld:pubmed |
| pubmed-article:19127523 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19127523 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19127523 | pubmed:pagination | 188-96 | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:meshHeading | pubmed-meshheading:19127523... | lld:pubmed |
| pubmed-article:19127523 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19127523 | pubmed:articleTitle | Involvement of R-type Ca2+ channels in neurotransmitter release from spinal dorsolateral funiculus terminals synapsing motoneurons. | lld:pubmed |
| pubmed-article:19127523 | pubmed:affiliation | Department of Physiology, Biophysics and Neuroscience, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City, CP 07300, Mexico. | lld:pubmed |
| pubmed-article:19127523 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19127523 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
