Source:http://linkedlifedata.com/resource/pubmed/id/19127523
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2009-2-2
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| pubmed:abstractText |
Molecular studies have revealed the presence of R-type voltage-gated Ca(2+) channels at pre- and postsynaptic regions; however, no evidence for the participation of these channels in transmitter release has been presented for the spinal cord. Here we characterize the effects of SNX-482, a selective R channel blocker, on the monosynaptic excitatory postsynaptic potentials (EPSPs) evoked in motoneurons by stimulation of dorsolateral funiculus (DLF) terminals in a slice preparation from the adult turtle spinal cord. SNX-482 inhibited neurotransmission in a dose-dependent manner, with an IC(50) of approximately 9 +/- 1 nM. The EPSP time course and membrane time constant of the motoneurons were not altered, suggesting a presynaptic mechanism. The toxin inhibited the residual component of the EPSPs recorded in the presence of N- and P/Q-type Ca(2+) channel blockers, strongly suggesting a role for the R channels in neurotransmission at the spinal cord DLF terminals. Consistently with this, RT-PCR analysis of turtle spinal cord segments revealed the expression of the Ca(V)2.3 pore-forming (alpha(1E)) subunit of R channels, whereas the use of anti-alpha(1E)-specific antibodies resulted in its localization in the DLF fibers as demonstrated by immunohistochemistry coupled with laser confocal microscopy.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1096-9861
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
10
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| pubmed:volume |
513
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
188-96
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| pubmed:meshHeading |
pubmed-meshheading:19127523-Analysis of Variance,
pubmed-meshheading:19127523-Animals,
pubmed-meshheading:19127523-Blotting, Western,
pubmed-meshheading:19127523-Calcium Channel Blockers,
pubmed-meshheading:19127523-Calcium Channels, R-Type,
pubmed-meshheading:19127523-Dose-Response Relationship, Drug,
pubmed-meshheading:19127523-Electric Stimulation,
pubmed-meshheading:19127523-Excitatory Postsynaptic Potentials,
pubmed-meshheading:19127523-Immunohistochemistry,
pubmed-meshheading:19127523-Microscopy, Confocal,
pubmed-meshheading:19127523-Motor Neurons,
pubmed-meshheading:19127523-Neural Inhibition,
pubmed-meshheading:19127523-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19127523-Spider Venoms,
pubmed-meshheading:19127523-Spinal Cord,
pubmed-meshheading:19127523-Synaptic Transmission,
pubmed-meshheading:19127523-Time Factors,
pubmed-meshheading:19127523-Turtles
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| pubmed:year |
2009
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| pubmed:articleTitle |
Involvement of R-type Ca2+ channels in neurotransmitter release from spinal dorsolateral funiculus terminals synapsing motoneurons.
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| pubmed:affiliation |
Department of Physiology, Biophysics and Neuroscience, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City, CP 07300, Mexico.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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