Source:http://linkedlifedata.com/resource/pubmed/id/19126717
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 1
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pubmed:dateCreated |
2009-1-7
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pubmed:abstractText |
A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27T, isolated from leaf tissues of rice (Oryza sativa L. 'Dong-Jin'), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae, Methylobacterium fujisawaense and Methylobacterium mesophilicum; strain CBMB27T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA-DNA hybridization experiments revealed low levels (<38 %) of DNA-DNA relatedness between strain CBMB27T and its closest relatives. The sequence of the 1-aminocyclopropane-1-carboxylate deaminase gene (acdS) in strain CBMB27T differed from those of close relatives. The major fatty acid of the isolate was C(18 : 1)omega7c and the G+C content of the genomic DNA was 66.8 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA-DNA hybridization, and physiological and biochemical characterization, which enabled the isolate to be differentiated from all recognized species of the genus Methylobacterium, it was concluded that strain CBMB27T represents a novel species in the genus Methylobacterium for which the name Methylobacterium phyllosphaerae sp. nov. is proposed (type strain CBMB27T =LMG 24361T =KACC 11716T =DSM 19779T).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-aminocyclopropane-1-carboxylate...,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon-Carbon Lyases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1466-5026
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
22-7
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pubmed:meshHeading |
pubmed-meshheading:19126717-Bacterial Typing Techniques,
pubmed-meshheading:19126717-Carbon-Carbon Lyases,
pubmed-meshheading:19126717-DNA, Bacterial,
pubmed-meshheading:19126717-DNA, Ribosomal,
pubmed-meshheading:19126717-Genes, rRNA,
pubmed-meshheading:19126717-Korea,
pubmed-meshheading:19126717-Methylobacterium,
pubmed-meshheading:19126717-Nucleic Acid Hybridization,
pubmed-meshheading:19126717-Oryza sativa,
pubmed-meshheading:19126717-Phenotype,
pubmed-meshheading:19126717-Phylogeny,
pubmed-meshheading:19126717-Pigmentation,
pubmed-meshheading:19126717-Plant Leaves,
pubmed-meshheading:19126717-RNA, Ribosomal, 16S,
pubmed-meshheading:19126717-Sequence Analysis, DNA,
pubmed-meshheading:19126717-Species Specificity
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pubmed:year |
2009
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pubmed:articleTitle |
Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice.
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pubmed:affiliation |
Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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