Linked Life Data

19125404

Source:http://linkedlifedata.com/resource/pubmed/id/19125404

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2009-4-9
pubmed:abstractText
The Wingless family of secreted proteins impinges on multiple aspects of vertebrate nervous system development, from early global patterning and cell fate decision to synaptogenesis. Here, we mapped the developmental expression of the Tcf7l2, which is key to the canonical Wingless signaling cascade, in the developing cerebellum. The exclusive and transient expression of Tcf7l2 in ventricular and Olig2-defined precursor cells within the cerebellar anlage, and its predominant expression in postmitotic neurons in the midbrain/inferior colliculus allowed us to ask whether cell type-specific differences are also reflected in splice isoform variability. We also included in this analysis intestinal epithelia, where Tcf7l2 function has been intensively studied. Our data reveal extensive variability of Tcf7l2 splicing in the central nervous system. Additional variability in brain-expressed Tcf7l2 is generated by a length polymorphism of expressed mRNAs in a stretch of normally nine adenines found at the beginning of exon 18, reminiscent of variability observed at the same site in cancers with microsatellite instability. A consensus emerging from our data is that the expression of isoforms comprising or lacking the C-clamp motif, which has been linked by in vitro studies to the regulation of cell growth, is indeed tightly correlated with the proliferative status in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1097-4547
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1532-46
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:19125404-Animals, pubmed-meshheading:19125404-Animals, Newborn, pubmed-meshheading:19125404-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:19125404-Cerebellum, pubmed-meshheading:19125404-Cerebrum, pubmed-meshheading:19125404-Gene Expression, pubmed-meshheading:19125404-Immunohistochemistry, pubmed-meshheading:19125404-In Situ Hybridization, pubmed-meshheading:19125404-Intestinal Mucosa, pubmed-meshheading:19125404-Mice, pubmed-meshheading:19125404-Mice, Inbred C57BL, pubmed-meshheading:19125404-Mitosis, pubmed-meshheading:19125404-Nerve Tissue Proteins, pubmed-meshheading:19125404-Neurogenesis, pubmed-meshheading:19125404-Neurons, pubmed-meshheading:19125404-Nuclear Proteins, pubmed-meshheading:19125404-Polymerase Chain Reaction, pubmed-meshheading:19125404-Polymorphism, Genetic, pubmed-meshheading:19125404-Protein Isoforms, pubmed-meshheading:19125404-RNA, Messenger, pubmed-meshheading:19125404-TCF Transcription Factors, pubmed-meshheading:19125404-Transcription Factor 7-Like 2 Protein
pubmed:year
2009
pubmed:articleTitle
Expression and molecular diversity of Tcf7l2 in the developing murine cerebellum and brain.
pubmed:affiliation
Anatomisches Institut, Anatomie and Zellbiologie, Rheinische-Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't