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pubmed-article:1912488pubmed:abstractTextPhytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.lld:pubmed
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pubmed-article:1912488pubmed:articleTitleIsolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana.lld:pubmed
pubmed-article:1912488pubmed:affiliationDepartment of Plant Pathology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.lld:pubmed
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