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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1991-11-21
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pubmed:databankReference |
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pubmed:abstractText |
Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0167-4412
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
609-14
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1912488-Amino Acid Sequence,
pubmed-meshheading:1912488-Base Sequence,
pubmed-meshheading:1912488-Blotting, Southern,
pubmed-meshheading:1912488-DNA Probes,
pubmed-meshheading:1912488-Molecular Sequence Data,
pubmed-meshheading:1912488-Multigene Family,
pubmed-meshheading:1912488-N-Glycosyl Hydrolases,
pubmed-meshheading:1912488-Oligonucleotides,
pubmed-meshheading:1912488-Plant Proteins,
pubmed-meshheading:1912488-Plants,
pubmed-meshheading:1912488-Ribosome Inactivating Proteins, Type 1,
pubmed-meshheading:1912488-Sequence Homology, Nucleic Acid
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pubmed:year |
1991
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pubmed:articleTitle |
Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana.
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pubmed:affiliation |
Department of Plant Pathology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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