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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2009-1-20
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pubmed:abstractText |
Aggregation of the high affinity IgE receptor (Fc epsilonRI) activates a cascade of signaling events leading to mast cell activation. Subsequently, inhibitory signals are engaged for turning off activating signals. We identified that regulator of calcineurin (Rcan) 1 serves as a negative regulator for turning off Fc epsilonRI-mediated mast cell activation. Fc epsilonRI-induced Rcan1 expression was identified by suppression subtractive hybridization and verified by real-time quantitative polymerase chain reaction and Western blotting. Deficiency of Rcan1 led to increased calcineurin activity, increased nuclear factor of activated T cells and nuclear factor kappaB activation, increased cytokine production, and enhanced immunoglobulin E-mediated late-phase cutaneous reactions. Forced expression of Rcan1 in wild-type or Rcan1-deficient mast cells reduced Fc epsilonRI-mediated cytokine production. Rcan1 deficiency also led to increased Fc epsilonRI-mediated mast cell degranulation and enhanced passive cutaneous anaphylaxis. Analysis of the Rcan1 promoter identified a functional Egr1 binding site. Biochemical and genetic evidence suggested that Egr1 controls Rcan1 expression. Our results identified Rcan1 as a novel inhibitory signal in Fc epsilonRI-induced mast cell activation and established a new link of Egr1 and Rcan1 in Fc epsilonRI signaling.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-10358778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-10719300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-10722714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-10861295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-10887340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-11100120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-12089510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-12360211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-12360215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-12543866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-12554096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-12560487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-1370575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-15640148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-15784689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-15928679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-15935327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-16231093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-16317093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-16415348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-16648267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-16894398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-17062574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-17595344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-17659377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-18056702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-18180251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-1991831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-7608202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-7678779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-7918637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-8112299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-8595418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-9681677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-9712046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19124655-9915860
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DSCR1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Dinitrofluorobenzene,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Egr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine,
http://linkedlifedata.com/resource/pubmed/chemical/trinitrophenyl-bovine serum albumin
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1540-9538
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
16
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pubmed:volume |
206
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
195-207
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:19124655-Animals,
pubmed-meshheading:19124655-Mice,
pubmed-meshheading:19124655-Hypersensitivity,
pubmed-meshheading:19124655-Mast Cells,
pubmed-meshheading:19124655-Serum Albumin, Bovine,
pubmed-meshheading:19124655-Muscle Proteins,
pubmed-meshheading:19124655-Cells, Cultured,
pubmed-meshheading:19124655-Protein Binding,
pubmed-meshheading:19124655-Mice, Inbred C57BL,
pubmed-meshheading:19124655-Cell Degranulation,
pubmed-meshheading:19124655-Signal Transduction,
pubmed-meshheading:19124655-Promoter Regions, Genetic,
pubmed-meshheading:19124655-Dinitrofluorobenzene,
pubmed-meshheading:19124655-Receptor Aggregation,
pubmed-meshheading:19124655-Gene Expression,
pubmed-meshheading:19124655-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:19124655-Cytokines,
pubmed-meshheading:19124655-Receptors, IgE,
pubmed-meshheading:19124655-Blotting, Western,
pubmed-meshheading:19124655-Mice, Knockout,
pubmed-meshheading:19124655-Early Growth Response Protein 1
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