Linked Life Data

19124021

Source:http://linkedlifedata.com/resource/pubmed/id/19124021

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-3-4
pubmed:abstractText
Two duplex real-time PCR assays were developed to diagnose three human parasites: Plasmodium falciparum, Plasmodium vivax and Plasmodium malariae. TaqMan duplex real-time PCR was evaluated in 263 blood samples of suspected malaria patients by comparing results against those obtained with microscopy and nested PCR. Compared with nested PCR, duplex real-time PCR assays showed 100% sensitivity and specificity. Duplex real-time PCR detected all mixtures of P. falciparum and P. vivax DNA, except at threshold detection limits for both parasites in which P. vivax was not amplified. Threshold detection limits of real-time PCR were 3.1, 0.3 and 0.8 parasites per microlitre of blood for P. falciparum, P. vivax and P. malariae, respectively. Duplex real-time PCR allows the detection of malarial cases, including mixed species infection, it simplifies analysis and reduces cost. Thus, this protocol may prove invaluable for use in the diagnosis of human infection, trial treatments and epidemiologic studies in which high-throughput analyses are often required.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1090-2449
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
346-51
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Multiplex real-time PCR detection of P. falciparum, P. vivax and P. malariae in human blood samples.
pubmed:affiliation
Laboratoire Hospitalo-universitaire de Parasitologie et Mycologie Médicale, Equipe EA3593, UFR de Médecine, Université des Antilles et de la Guyane, Cayenne, French Guiana. vincent.veron@guyane.univ-ag.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't