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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-2-13
pubmed:abstractText
We have already shown the regulated expressions of hyaluronan binding molecules including versican, SPACR, and SPACRCAN, during retinal development. Here we analyzed the profiles of hyaluronan during these periods. Hyaluronan and hyaluronan synthase (HAS) expressions in chicken retinas at different ages were assessed by slot blot and real-time PCR, respectively. The molecular size of hyaluronan was determined using a Superose-6 column, and histochemistry was used to localize hyaluronan expression. Hyaluronan degradation activity was measured after adding hyaluronan to retinal extracts. In the embryonic retina, hyaluronan was mainly present in the inner plexiform and photoreceptor layers. Expression in the inner plexiform layer decreased with development and aging while expression in the photoreceptor layer remained constant. The molecular size of hyaluronan decreased from embryonic day 12 (E12) to postnatal day 1 (P1) with increasing retinal hyaluronan degradation activity. In adult retinas, hyaluronan was only present in the photoreceptor layer, and its molecular size was the highest. HAS-2 and HAS-3 expressions increased from E15 to P1, but both were reduced in adulthood. Thus hyaluronan was specifically detected where versican, SPACR, and SPACRCAN were previously demonstrated, and the synthesis and degradation of hyaluronan are regulated. Hyaluronan together with versican, SPACR, and SPACRCAN are closely related during retinal development, and may be important for retinal physiology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1872-6240
pubmed:author
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
1256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-60
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Hyaluronan dynamics during retinal development.
pubmed:affiliation
Department of Ophthalmology, Aichi Medical University, 21 Yazako, Karimata, Nagakute-cho, Aichi 480-1195, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't