Linked Life Data

19123483

Source:http://linkedlifedata.com/resource/pubmed/id/19123483

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2009-3-3
pubmed:abstractText
Phloretin (Ph), a natural product found in apples and pears with glucose transporter (GLUT) inhibitory activity, exerts antitumor effects. However, little is known about its effects on human liver cancer. The purpose of this study is to test the cytotoxic effects of Ph on HepG2 cells and to identify the underlying molecular pathways. Human hepatocellular carcinoma specimens and HepG2 show a high level of GLUT2 transporter activity in the cell membrane. Real-time PCR and MTT assays demonstrate that Ph-induced cytotoxicity correlates with the expression of GLUT2. Flow cytometry and DNA fragmentation studies show that 200 microM Ph induces apoptosis in HepG2, which was reversed by glucose pretreatment. GLUT2 siRNA knockdown induced HepG2 apoptosis, which was not reversed by glucose. Western blot analysis demonstrates that both intrinsic and extrinsic apoptotic pathways in addition to Akt and Bcl-2 family signaling pathways are involved in Ph-induced cell death in HepG2 cells. Furthermore, using flow cytometry analysis, a mitochondrial membrane potential assay and Western blot analysis, we show that cytochalasin B, a glucose transport inhibitor, enhances the Ph-induced apoptotic effect on HepG2 cells, which was reversed by pretreatment with glucose. Furthermore, we found significant antitumor effects in vivo by administering Ph at 10 mg/kg intraperitoneally to severe combined immune deficiency mice carrying a HepG2 xenograft. A microPET study in the HepG2 tumor-bearing mice showed a 10-fold decrease in (18)F-FDG uptake in Ph-treated tumors compared to controls. Taken together, these results suggest that Ph-induced apoptosis in HepG2 cells involves inhibition of GLUT2 glucose transport mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1097-0215
pubmed:author
pubmed:copyrightInfo
(c) 2008 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2210-9
pubmed:meshHeading
pubmed-meshheading:19123483-Animals, pubmed-meshheading:19123483-Apoptosis, pubmed-meshheading:19123483-Blotting, Western, pubmed-meshheading:19123483-Carcinoma, Hepatocellular, pubmed-meshheading:19123483-Caspases, pubmed-meshheading:19123483-Cell Proliferation, pubmed-meshheading:19123483-Cells, Cultured, pubmed-meshheading:19123483-Cytochalasin B, pubmed-meshheading:19123483-Flow Cytometry, pubmed-meshheading:19123483-Fluorescent Antibody Technique, pubmed-meshheading:19123483-Glucose, pubmed-meshheading:19123483-Glucose Transporter Type 2, pubmed-meshheading:19123483-Hepatocytes, pubmed-meshheading:19123483-Humans, pubmed-meshheading:19123483-Immunoenzyme Techniques, pubmed-meshheading:19123483-Liver Neoplasms, pubmed-meshheading:19123483-Male, pubmed-meshheading:19123483-Membrane Potential, Mitochondrial, pubmed-meshheading:19123483-Mice, pubmed-meshheading:19123483-Mice, SCID, pubmed-meshheading:19123483-Phloretin, pubmed-meshheading:19123483-Positron-Emission Tomography, pubmed-meshheading:19123483-RNA, Messenger, pubmed-meshheading:19123483-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19123483-Survival Rate, pubmed-meshheading:19123483-Transplantation, Heterologous
pubmed:year
2009
pubmed:articleTitle
In vitro and in vivo study of phloretin-induced apoptosis in human liver cancer cells involving inhibition of type II glucose transporter.
pubmed:affiliation
Graduate Institute of Clinical Medicine, Taipei Medical University Hospital, Taipei Medical University, Taipei, Taiwan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't