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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3-4
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pubmed:dateCreated |
2009-3-6
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pubmed:abstractText |
We now demonstrate that NAC1 acts as a corepressor for other POZ/BTB proteins. NAC1 is a POZ/BTB motif containing transcriptional repressor protein. In a mammalian two hybrid assay in neuronal (N2A) cells and non-neuronal (HEK 293T) cells, VP16 activation domain tagged NAC1 resulted in significant reversal of transcriptional inhibition with the Gal4-ZID, Gal4-BCL6, Gal4-ZF5, and kelch proteins Gal4-MAYVEN and Gal4-NRP/B fusion proteins. We also observed similar results with another corepressor, BCoR Gal4 fusion protein. NAC1 potentiated ZF5 mediated repression in Gal4-DBD fusion transient assays. GST pulldown assays further confirmed protein-protein interactions between these proteins and NAC1. Both the NAC1 isoforms demonstrated selective interaction through the POZ/BTB domain but not with the non-POZ/BTB region. Endogenous NAC1 and BCL6 physically associated in CNS regions. Strikingly, NAC1 did not interact with the pro-myelocytic leukemia zinc finger protein (PLZF), another POZ/BTB protein that is not found in the adult brain. Therefore, we conclude that NAC1 functions as a corepressor for POZ/BTB proteins expressed in the mature CNS.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BCOR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kruppel-Like Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/NACC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nacc1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ZBTB16 protein, human
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pubmed:status |
MEDLINE
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pubmed:issn |
1872-9754
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
245-52
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pubmed:dateRevised |
2011-11-3
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pubmed:meshHeading |
pubmed-meshheading:19121354-Animals,
pubmed-meshheading:19121354-Cell Line,
pubmed-meshheading:19121354-Central Nervous System,
pubmed-meshheading:19121354-Genes, Reporter,
pubmed-meshheading:19121354-Humans,
pubmed-meshheading:19121354-Kruppel-Like Transcription Factors,
pubmed-meshheading:19121354-Mice,
pubmed-meshheading:19121354-Neoplasm Proteins,
pubmed-meshheading:19121354-Nerve Tissue Proteins,
pubmed-meshheading:19121354-Protein Isoforms,
pubmed-meshheading:19121354-Protein Structure, Tertiary,
pubmed-meshheading:19121354-Proto-Oncogene Proteins,
pubmed-meshheading:19121354-Recombinant Fusion Proteins,
pubmed-meshheading:19121354-Repressor Proteins,
pubmed-meshheading:19121354-Transcription, Genetic
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pubmed:articleTitle |
NAC1, a POZ/BTB protein that functions as a corepressor.
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pubmed:affiliation |
Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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